Picture from Cui Y et al. (2009) J Biol Chem "Cadmium induces retinoic acid signaling by regulating retinoic acid metabolic ...."

Figure 1. bcmo-1::GFP expression in untreated C. elegans embryos. A, ~125 min post-fertilization; B, ~200 min post-fertilization; C, ~700 min post-fertilization. Embryos were obtained from nematodes not exposed to cadmium (magnification, 1200x).

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Lin X et al. (2003) Curr Biol "C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin ...."

PAT-3 in wild-type 530 min embryos. Arrows indicate body wall muscle attachments.

Picture from Lin X et al. (2003) Curr Biol "C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin ...."

UNC-89 in wild-type 530 min embryos. Arrows indicate body wall muscle attachments.

Picture from Updike DL et al. (2006) PLoS Genet "Temporal regulation of foregut development by HTZ-1/H2A.Z and ...."

Expression of R07B1.9::GFP at 90 and 180 min after the comma stage. Pharyngeal R07B1.9::GFP is indicated by arrowheads.

Picture from Polley SR et al. (2014) Genetics "Implicating SCF complexes in organogenesis in Caenorhabditis elegans."

Figure 4 Embryonic expression pattern of SUP-36::GFP. (A-F) Confocal fluorescence images of full-length functional SUP- 36::GFP in embryos. A and B show embryos prior to morphogenesis (100-350 min postfertilization), C and D show embryos at early stages of morphogenesis (350-400 min), E shows an embryo at the 1.5-fold stage (430 min), and F shows an 3-fold-stage embryo (.600 min). Arrows in A, B, and E indicate the positions of nuclei. Arrowheads in C indicate expression of SUP-36:: GFP along the basal surface of primordial pharyngeal cells in the region abutting the basement membrane. Dotted outlined regions indicate the developing pharynx. Bar in A, 10 mm for A-F.

Picture from Sullivan-Brown JL et al. (2016) Genetics "Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube ...."

Figure 5 (A) Ea/Ep cell cycle times are reduced in some sptf-3 (RNAi) embryos. In wild-type embryos, the Ea cell cycle (left column) is 40.33 6 0.39 min, and the Ep cell cycle (right column) is 42.64 6 0.39 min. In sptf-3(RNAi) embryos, the cell cycle of the Ea and Ep cells is reduced to 37.28 6 0.65 and 39.51 6 0.57 min, respectively. Ea and Ep cell cycle timing is not changed in abi-1(RNAi), gex-3(RNAi), and ced-4 (RNAi) embryos. **P , 0.01. Excluded is one abi-1(RNAi) embryo that had an anomalous delay outside the range shown here (Ea = 62 min; Ep = 64 min). Cells that failed to internalize are shown in red. Gray indicates cells that internalized. Means are represented by thick black bars. (B) mNeonGreen::SPTF-3 is enriched in all nuclei during gastrulation (nine of nine embryos filmed). Arrowheads mark Ea/Ep nuclei. Bar, 10 um.

Picture from Chan SS-Y et al. (1996) Cell "UNC-40, a C. elegans homolog of DCC (Deleted in Colorectal Cancer), is required in ...."

Figure 4. Fluorescence Micrographs of UNC-40/GFP Expression in Transgenic Animals. (A-D) Embryos of successive stages. Anterior is shown to the right. (A) Early gastrula (100 min) showing ubiquitous GFP expression.(B) Longer exposure of late gastrula (260 min) showing decreased expression in the posterior embryo.(C) Neurula (430 min) showing expression in ventral cord motorneurons (between arrows).(D) Neurula (460 min) showing first axons from interneurons growing into the ventral nerve cord from the head (arrowhead).(E and F) Late-stage larvae. (E) Ventral aspect showing axons (arrowheads) of embryonic motorneurons DB4, DD3, and DD4. Additional motorneurons (not labeled) are visible alongside the main axon fascicle. (F) Lateral aspect showing sensory neurons PDEL and PVDL, motor axons (arrowheads), and excretory canal (ec). Scale bar, 10 um.

Picture from Robinson B et al. (2019) MicroPubl Biol

Figure 1. UNC-108 is required for stress-induced sleep (SIS) and acts downstream of EGF signaling. (A,B) Compared to wild-type N2, unc-108(lf) mutants are defective for SIS induced by Cry5B toxin or UV light (P< 0.0001, one-way ANOVA with Dunnett's multiple comparisons test). Animals were exposed to Cry5B-expressing bacteria as described (Hill et al., 2014) and examined 20 min later, or exposed to UV radiation as described (Goetting et al., 2017) and examined 60 min later. These time points have been shown to be associated with robust ALA-dependent quiescence (Hill et al., 2014; Goetting et al., 2018). (C) LIN-3/EGF overexpression induces sleep in wild type but not in unc-108(csn2) animals (P< 0.0001, two-tailed Fisher's exact test).EGF overexpression from a hs:lin-3 transgene (Van Buskirk and Sternberg, 2007) was induced by mild heat shock (33C for 10 min) and animals were examined 60 min later for quiescence. In all panels, quiescence was defined as a complete cessation of locomotion, head movement and pharyngeal pumping during a 5 sec examination on a stereomicroscope. Mean and SD of three independent trials are shown, and each data point represents one trial of 25 well-fed young adult animals.

Picture from Robinson, Bryan et al. (2019) MicroPubl Biol

Figure 1 The RIS interneuron promotes head movement quiescence but not locomotor quiescence during lethargus and stress-induced sleep. (A-D) Locomotor rate assessed by body bends per minute among animals during the L4-to-adult lethargus (A), during the young adult stage (B), and during stress-induced sleep at the young adult stage following exposure to ultraviolet light (C) or Cry5B pore-forming toxin (D). Animals were examined on thin even lawns of E. coli OP50 and examined for the number of sinusoidal body bends (peak-to-peak movement of any body part) in one minute. To select mid-lethargus animals, late-L4 larvae were identified based on vulval morphology, screened one hour later for cessation of pharyngeal pumping as an indicator of lethargus entry, and scored 30 min later for bend rates. For UV-SIS, worm plates were placed lid-down on a 302nm 60mW UV light box for 50 sec and examined 60 min later. For Cry5B-SIS, animals were exposed to Cry5B-expressing bacteria for 5 min, transferred to a thin even lawn of OP50, and examined 10-15 min later. For all bend rate analyses, plates were gently moved to the stereomicroscope field of view and allowed to sit for 1 min prior to examination. Each animal was examined for 1 min and a body bend was defined as a peak-to-peak unit of sinusoidal motion. **P<0.0001 vs. N2, one-way ANOVA with Dunnett's multiple comparisons test. N=15-20, experimenter blind to genotype. (E-L) Categorization of movement during SIS following UV exposure (E-H) and during exposure to Cry5B (I-L). Each animal was examined for 5 sec and characterized as completely immobile (E and I), head mobile but body immobile (F and J), or head mobile plus body movement of at least 1/10 body-length (G and K). Two-way repeated measures (RM) ANOVA with time as the repeated factor and Sidak's multiple comparisons test reveals that more ceh-17(lf) than aptf-1(lf) animals are completely immobile under certain SIS conditions (Panel I, ceh-17 vs. each aptf-1 mutant, P<0.05 at 40 min). However, aptf-1 mutants are impaired for head movement quiescence (N2 vs. aptf-1 mutants P<0.05 at multiple time points during UV-SIS and Cry5B-SIS), and not significantly impaired for body movement with the exception of the 30 min time point of UV-SIS, for which aptf-1(tm3287) is different from wild type N2 (P<0.05). H and L show stacked categories of behavior for the time points indicated by shaded boxes within the time courses. Three trials of 25 animals were performed and the experimenter was blind to genotype.