Picture from O'Hagan R et al. (2011) Curr Biol "The tubulin deglutamylase CCPP-1 regulates the function and stability of ...."

(D) In adult hermaphrodites, CCPP-1::GFP was expressed in amphid and IL2 core ciliated sensory neurons. A cilium containing CCPP-1::GFP is indicated. (E) Confocal projections of CCPP-1::GFP expression in males. Top (L4): CCPP-1::GFP was expressed in (left) amphid, IL2, and CEM neurons and (right)male tail ray and HOB neuronal cell bodies (empty arrowheads). Gubernacular erector (g.e.) and retractor (g.r.) muscles are indicated. Bottom (1-day-old adult): CCPP-1::GFP localization in ray neuron dendrites (arrow) and a cilium (arrowhead) is shown.

Picture from O'Hagan R et al. (2011) Curr Biol "The tubulin deglutamylase CCPP-1 regulates the function and stability of ...."

Figure 1. CCPP-1 Is Required for PKD-2 Localization and Is Expressed in the Ciliated Sensory Nervous System (A) Diagram of the male-specific CEM neurons in the head and hook B-type (HOB) and RnB (ray B-type, where n = 1-9, excluding 6) neurons in the tail. Box illustrates the region of CEM (cephalic sensilla, male) cilia and distal dendrites shown in the epifluorescent images. In the ventral-up cartoon of the male tail, the RnBs innervate the tail rays; R3B dendrite is shown as an example. (B) In wild-type males, PKD-2::GFP faintly illuminated cilia of the CEM neurons, the HOB cilium, and the cilia of the RnB neurons. ccpp-1(my22) and ccpp- 1(ok1821) mutants exhibit the Cil phenotype. Arrows point to CEM cilia; arrowheads point to R1B and R2B cilia. Expression of genomic ccpp-1 rescued the Cil phenotype of ccpp-1(ok1821). (C) CCPP-1 contains several conserved regions, including a zinc carboxypeptidase domain, an aspartic-acid rich domain, a conserved 'NT' region (N-terminal to the carboxypeptidase domain; [12]), and predicted NLS (nuclear localization signal; [36]). CCPP-1 also contains a predicted leucine zipper near the N terminus (ScanProsite [37]). The my22 lesion affects the conserved sequence FESGNL in the NT region of CCPP-1. (D) In adult hermaphrodites, CCPP-1::GFP was expressed in amphid and IL2 core ciliated sensory neurons. A cilium containing CCPP-1::GFP is indicated. (E) Confocal projections of CCPP-1::GFP expression in males. Top (L4): CCPP-1::GFP was expressed in (left) amphid, IL2, and CEM neurons and (right)male tail ray and HOB neuronal cell bodies (empty arrowheads). Gubernacular erector (g.e.) and retractor (g.r.) muscles are indicated. Bottom (1-day-old adult): CCPP-1::GFP localization in ray neuron dendrites (arrow) and a cilium (arrowhead) is shown. (F) ccpp-1 mutants are defective in response behavior. Number of trials and number of males for each genotype was as follows: wild-type, seven trials, n = 70 males; ok1821, six trials, n = 60; ok1821;Ex[ccpp-1(+)], three trials, n = 29; ok1821;Ex[ccpp-1::gfp], three trials, n = 24;my22, four trials, n = 40; my22;Ex[ccpp- 1::gfp], five trials, n = 39. Error bars indicate standard error of the mean (SEM); ** indicates that ccpp-1 mutants were statistically different (p < 1024, analysis of variance [ANOVA]/Tukey HSD test) from mutants expressing ccpp-1 transgenes, which were similar to wild-type. See also Figure S1.

Picture from Susoy, Vladislav et al. (2019) MicroPubl Biol

Figure 1. Psto3::wrmScarlet reporter is expressed in R4BL/R and R8BL/R ray neurons.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

The srd-33 7TMR reporter gene fusion also shows a restricted expression pattern (ASEL/R, AWBL/R, ASHL/R, AVKL/R, PHAL/R, PHBL/R, and weakly in another unidentified tail neuron pair, possibly PHCL/R). Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp.

Picture from Lin L et al. (2013) J Cell Biol "The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic ...."

At larval stages, W07G4.5::GFP forms aggregates in intestinal cells (Q and R).

Picture from Yan D et al. (2009) Cell "The DLK-1 kinase promotes mRNA stability and local translation in C. elegans ...."

Figure S7. Colocalization analysis of MAK-2 and CEBP-1 with presynapticmarkersAll images are of synaptic regions of the dorsal processes of GABAergic motorneurons. For each panel, pixel values obtained from line scans of a confocalprojection (on left) are plotted on the right. Pearson's correlation coefficient r and the probability were calculated. (A) mCherry::MAK-2 (juEx1503) partly colocalizes with SNB-1::GFP (juIs1). r = 0.69, P < 0.0001. (B) In rpm-1(lf) mutants mCherry::MAK-2 and SNB-1::GFP are aberrantly distributed but remain colocalized. (C) mCherry::MAK-2 does not colocalize with RPM-1::GFP (juIs77). r = -0.14, P = 0.054. (D) mCherry::CEBP-1 (juEx1735) colocalizes with SNB1::GFP. r = 0.88, P < 0.0001.(E) mCherry::CEBP-1 is excluded from areas of RPM-1::GFP. r = -0.67, P < 0.001. (F) mCherry::MAK-2 and CEBP-1::GFP (juEx2157) partly colocalize; r = 0.48, P < 0.0001. Scale, 10 um.

Picture from Mariner BL et al. (2023) MicroPubl Biol "Easy C. elegans Worm Lifespan Plotting and Statistics with ...."

Figure 1. Example plot generated from WLSplot workflow:Visualization of WLS_autoplot() features when plotting worm lifespan data with the WLSplot R package.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

Figure 2. Expression patterns of genes from the SAGE library as assessed by gfp reporter gene technology. Only a selected and representative number of examples are shown in this figure. All analyzed reporter strains, including those shown here, are shown in Supplementary Figures 1-3. Green boxes indicate gfp coding sequences (rfp in the case of dop-3 and yfp in the case of hlh-10). If extrachromosomal arrays were generated, multiple lines were analyzed for each reporter construct, as indicated below each panel of micrographs. Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp. (A) Expression patterns of two selected TFs from the SAGE data set. (B) Expression patterns of two selected peptidergic signaling proteins from the SAGE data set. (C) Expression patterns of two selected putative chemoreceptor proteins of the 7TMR family from the SAGE data set. The srg-30 7TMR-type chemoreceptor shows the most restricted expression pattern with strong expression in ASEL/R and weak expression in the chemosensory neurons ASIL/R and ADLL/R. Additional expression can be observed in a pharyngeal neuron. The srd-33 7TMR reporter gene fusion also shows a restricted expression pattern (ASEL/R, AWBL/R, ASHL/R, AVKL/R, PHAL/R, PHBL/R, and weakly in another unidentified tail neuron pair, possibly PHCL/R). The bottom panels in C demonstrate that 7TMR-type chemoreceptors localize to the dendritic ending of the ASE neurons. In order to boost expression in the ASE neurons, a 188-bp ASEL-specific regulatory element from the gcy-7 gene was appended to each locus (indicated as a black box). One transgenic line each was analyzed in detail. Yellow arrows point to the dendritic endings.

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

The srg-30 7TMR-type chemoreceptor shows the most restricted expression pattern with strong expression in ASEL/R and weak expression in the chemosensory neurons ASIL/R and ADLL/R. Additional expression can be observed in a pharyngeal neuron. Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp.