Picture from Nonet ML et al. (1998) J Neurosci "Synaptic transmission deficits in Caenorhabditis elegans synaptobrevin ...."

Expression of snb-1 in the C. elegans nervous system. Whole worms fixed and stained with a-SNB-1 primary antibodies. A, Lateral view of the head region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the nerve ring, pharyngeal nervous system, and ventral and dorsal nerve cords. B, Ventral view of the tail region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the ventral nerve cord.

Picture from Nonet ML et al. (1998) J Neurosci "Synaptic transmission deficits in Caenorhabditis elegans synaptobrevin ...."

G, H, Transgenic jsEx96 animals expressing the psnb-1::lacZ reporter construct pSB111 stained for b-galactosidase activity, using X-gal. lacZ contains an SV40 nuclear localization signal. G, Lateral view of the head region of an adult hermaphrodite showing staining neurons in the head ganglia and pharynx. H, Lateral view of the vulval region of an adult hermaphrodite showing staining in the motor neurons in the ventral cord and two neurons in the deirid sensillum.

Picture from Nonet ML et al. (1998) J Neurosci "Synaptic transmission deficits in Caenorhabditis elegans synaptobrevin ...."

Figure 2. Expression of snb-1 in theC. elegans nervous system. A-F, Whole worms fixed and stained with a-SNB-1, a-RAB-3, and a-UNC-64 (syntaxin) primary antibodies and visualized with FITC- or Cy3-conjugated antibodies. A, Lateral view of the head region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the nerve ring, pharyngeal nervous system, and ventral and dorsal nerve cords. B, Ventral view of the tail region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the ventral nerve cord. C, D, Ventral view of the midsection of a wild-type adult hermaphrodite showing colocalization of SNB-1 (C) and RAB-3 (D) immunoreactivity in the ventral nerve cord.E, Ventral view of the head of SNB-1 immunoreactivity in an adult snb-1(md247) mutant. The SNB-1 mutant protein remains limited to the synaptic regions of the SAB neurons.F, Similar view of UNC-64 syntaxin immunoreactivity in a wild-type animal reveals all processes in the head. G, H, Transgenic jsEx96 animals expressing thep snb-1:: lacZreporter construct pSB111 stained for b-galactosidase activity, using X-gal. lacZ contains an SV40 nuclear localization signal. G, Lateral view of the head region of an adult hermaphrodite showing staining neurons in the head ganglia and pharynx.H, Lateral view of the vulval region of an adult hermaphrodite showing staining in the motor neurons in the ventral cord and two neurons in the deirid sensillum.

Picture from Nonet ML et al. (1997) J Neurosci "Caenorhabditis elegans rab-3 mutant synapses exhibit impaired function and are ...."

Figure 2. RAB-3 expression in the C. elegans nervous system. Whole worms were fixed and stained with anti-RAB-3 and anti-synaptotagmin primary antibodies and visualized with FITC- or Cy3-conjugated antibodies. A, B, Lateral view of the head region of a wild-type adult hermaphrodite showing RAB-3 (A) and synaptotagmin (B) immunoreactivity in the nerve ring (NR), pharyngeal nervous system (PN), and SAB neuron axonal processes (arrows). C, D, Ventral view of the midsection of an adult hermaphrodite showing RAB-3 (C) and synaptotagmin (D) immunoreactivity in the ventral nerve cord (VC;arrow) and the ventral sublateral processes.E, F, Lateral view of the vulval region of an adult hermaphrodite illustrating the absence of RAB-3 (E) but the presence of synaptotagmin (F) immunoreactivity in the uv1 cells of the somatic gonad (uv1; arrow). The ventral cord is also visible (VC).

Picture from Nonet ML et al. (1999) Mol Biol Cell "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and ...."

Figure 4. UNC-11 is enriched in synaptic regions of C. elegans. Confocal images of adult nematodes prepared for immunohistochemistry using both affinity-purified rabbit anti-UNC-11 antisera and affinity-purified mouse anti-synaptotagmin antisera. Animals were double stained for UNC-11 with rhodamine-conjugated secondary antibody and for synaptotagmin with FITC-conjugated secondary antibody: wild-type (A-E), unc-11(e47) (F and G), and unc-11 (n2954) (H and I). Anterior is left in all images. Panel C is the merge of panels A and B. A representative nerve ring, ventral nerve cord (V. N. C.), dorsal nerve cord (D. N. C.), and coelomocyte are labeled in panel D. Insets in panels A-C and H de- pict localization of UNC-11 staining in neuronal cell bodies. Bar, 10 um.

Picture from Nonet ML et al. (1999) Mol Biol Cell "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and ...."

UNC-11 is enriched in synaptic regions of C. elegans. Confocal images of adult nematodes prepared for immunohistochemistry using both affinity-purified rabbit anti-UNC-11 antisera and affinity-purified mouse anti-synaptotagmin antisera. Animals were double stained for UNC-11 with rhodamine-conjugated secondary antibody and for synaptotagmin with FITC-conjugated secondary antibody: wild-type (A-E). Anterior is left in all images. Panel C is the merge of panels A and B. A representative nerve ring, ventral nerve cord (V. N. C.), dorsal nerve cord (D. N. C.), and coelomocyte are labeled in panel D. Insets in panels A-C depict localization of UNC-11 staining in neuronal cell bodies. Bar, 10 um.

Picture from Nonet ML et al. (1999) Mol Biol Cell "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and ...."

Subcellular localization of UNC-11 in wild-type nematodes. Confocal images of freeze-cracked nematodes double stained with affinity-purified rabbit anti-UNC-11 anti-sera (first row); affinity purified mouse anti-synaptotagmin antisera (second row), and merge (third row). (A-C) Wild- type. A diagram depicting the organization of nervous tissue in the C. elegans head including the position of neuronal cell bodies, the nerve ring neuropil, and the ventral and dorsal nerve cords is positioned above the confocal images. Synaptic-rich regions are depicted in red. Commissures, dendrites, minor process bundles, and many neuronal cell bodies of the ganglia were omitted for clarity. Bar, 10 um.

Picture from Nonet ML et al. (1999) Mol Biol Cell "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and ...."

Figure 5. Subcellular localization of UNC-11 in wild-type and unc-104 mutant nematodes. Confocal images of freeze-cracked nematodes double stained with affinity-purified rabbit anti-UNC-11 anti-sera (first row); affinity purified mouse anti-synaptotagmin antisera (second row), and merge (third row). (A-C) Wild- type. (D-F) unc-104(rh43). A diagram depicting the organization of nervous tissue in the C. elegans head including the position of neuronal cell bodies, the nerve ring neuropil, and the ventral and dorsal nerve cords is positioned above the confocal images. Synaptic-rich regions are depicted in red. Commissures, dendrites, minor process bundles, and many neuronal cell bodies of the ganglia were omitted for clarity. Bar, 10 um.

Picture from Miller DM et al. (1983) Cell "Differential localization of two myosins within nematode thick ...."

Figure 10. Antibody S-8 Reacts with the Central Region of the Thick Filament Only(A) Electron micrograph of negatively stained thick filament. (B) Low, (C) medium, and (D) high magnification images of a thick filament reacted with antibody 5-8 (1 m/ml) plus goat antimouse IgG (20 ug/ml). Pointers denote edges of antibody reacted zone. Bars in (A, B) are 1 um and bars in (C, D) are 0.2 um.

Picture from Kullyev A et al. (2010) Genetics "A genetic survey of fluoxetine action on synaptic transmission in ...."

Figure 1.Fluoxetine treatment and mutations in mod-5/ SERTabolish anti-5-HT immunoreactivity in the RIH and AIM neurons. (a-d) Photomicrographs of animals stained with anti-5-HT antibodies. To test the effects of MOD-5/SERT inhibitors, WT animals were incubated overnight on NGM plates containing 0.5 mg/ml of fluoxetine (as shown) or 0.4 mg/ml of imipramine (not shown) before fixation for staining. (e and f) Photomicrographs of WT animals expressing tph-1Tgfp. All the animals shown have anterior to the right.