Figure 3. Expression of
mig-17. (A, C, E, G, andI) GFP fluorescence; (B, D,F, H, and J) Nomarski. Same animal in each left-right pair. Photos show ventral view (A and B) and lateral view of posterior gonad arms (C through J). (A through F)
mig-17(
k174) with mig- 17::GFP; (G and H)
unc-119(
e2498) with mig- 17::GFP and pDP#MM016B (
unc-119); (I and J)
unc-119(
e2498) with mig-17EA:GFP and pDP#MM016B. (A and B) A young adult hermaphrodite. GFP is expressed on the pseudocoelomic face of ventral body wall muscles, but not on the ventral hypodermal ridge (arrowheads). (C and D) A mid L3 hermaphrodite. The DTC (arrow) starts turning dorsally. GFP is not expressed on the surface of gonad. The dotted fluorescence results from the auto-fluorescence of gut granules. (E and F) A late L3 hermaphrodite. The DTC migrates toward the dorsal muscle cells. GFP is expressed on the surface of gonad. [Frequency of GFP detection on the gonad: 0% (n = 12) during migration on ventral muscle; 44% (n = 9) during migration on lateral hypodermis; 90% (n = 21) during migration on dorsal muscle]. (G through J) Mid L4 hermaphrodites. MIG-17::GFP is expressed on the surface of the gonad, but MIG-17EA::GFP is not. Bar, 20 um.