Mutations in
hda-4 suppress the extended lifespan of
kin-29 mutants. Lifespan was determined by assaying the percent survival of animals as a function of time. Scoring was performed every other day on NGM plates containing 5 uM FUDR and concentrated E. coli OP50 bacteria (see methods). Mean lifespans were with n>100 animals for each genotype (days +/- SEM): wild-type,
kin-29(
oy38) and
hda-4(
oy57) single mutants, and
kin-29(
oy38)
hda-4(
oy57) double mutants. Data is shown from a single biological repeat (#4) with all strains examined in parallel. The mean lifespan of
hda-4 single and
kin-29hda-4 double mutants is significantly different from that of wild-type and
kin-29 single mutants, whereas the mean lifespan of
hda-4 single mutants is not statistically different from
kin-29hda-4 double mutants (log-rank test, p<0.01). * indicates animals fed at a higher food density. See table for data of each biological repeat and a summary of lifespan statistics.