Fig 2. ZHP-4 and ZHP-3 colocalize and are interdependent for their localization to the SC and crossover sites. (A) alpha-ZHP-4(green) and alpha-ZHP-3 (red) immunostaining in wild-type pachytene stage nuclei showing colocalization with the SC in early and mid-pachytene followed by co-restriction to ~6 distinct foci per nucleus in late pachytene. (B) Immunostainings of midpachytene region nuclei of the indicated genotypes with alpha-ZHP-4 (green) and alpha-ZHP-3 (red). ZHP-4 could not be detectedabove background levels in
zhp-3(
jf61) germlines stained with DAPI (blue) and conversely no ZHP-3 could be detected in
zhp-4(
vv103) mutants. Scale bars 5 um.