Picture from Newbury S et al. (2004) RNA "The 5'-3' exoribonuclease xrn-1 is essential for ventral epithelial enclosure ...."

FIGURE 3. (A) (Panels A, B) Expression pattern of xrn-1GFP. GFP fluorescence pattern of animals carrying a rol-6 marker and a GFP reporter fused to the second exon of xrn-1, expressed as a nonintegrated extrachromosomal array. (B) Effect of xrn-1 on RNA interference. Panels A and B: Expression of an integrated histone GFP marker in the nuclei of oocytes (A) and embryos (B) in the germline of wild-type hermaphrodites. Panel C: histoneGFP hermaphrodites injected with double-stranded GFP RNA showing complete silencing of GFP expression, 48 h postinjection. Panel D: Co-injection of dcr-1 and GFP double-stranded RNA into hermaphrodites showing faint fluorescence in oocytes. Panel E: Silencing of GFP expression in adult worms co-injected with double-stranded GFP RNA and double-stranded unc-22 RNA 48 h postinjection. Panel F: histoneGFP hermaphrodites co-injected with xrn-1 and GFP double-stranded RNA showing faint fluorescence in the oocyte nuclei of the germline 48 h after gonad injection. Panel G: Triple injection of double-stranded xrn-1, dcr-1, and GFP RNA into hermaphrodites results in faint fluorescence in oocytes in most worms. Panel H: Control triple injection of unc-22, mab-9, and GFP double-stranded RNA showing no fluorescence except for autofluorescence in the gut. Scale bar, 50 um.

Picture from Timbers TA et al. (2016) PLoS Genet "Accelerating Gene Discovery by Phenotyping Whole-Genome Sequenced ...."

B) ADL cilia correctly enter the amphid socket (AMso) cells in bgnt-1.1 mutants. ADL cilia are labelled with Psrh-220::IFT-20::tdTomato. IFT-20(IFT20) localises to cilia basal bodies (bb) and axonemes. The srh-220 promoter drives expression primarily in ADL neurons. The amphid socket (AMso) cells are labelled with cytoplasmic GFP driven by an amphid socket specific promoter, grd-15 (Hunt-Newbury et al. 2007).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)). (C) Overexpression of UNC-33(S) suppresses unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.

Picture from Ellefson ML et al. (2009) Mol Biol Cell "Kinesin-1 and cytoplasmic dynein act sequentially to move the meiotic spindle to ...."

Images of mCherry-histone (top row) and GFP: DHC-1 (bottom row) within a wild-type meiotic embryo are shown from a representative time-lapse sequence. The cortex has been highlighted in each image for clarity. 0 s is the start of rotation. GFP:DHC-1 was faintly localized across the meiotic spindle before spindle shortening (T = -120 s). 45-30 s before the start of spindle rotation, DHC-1:GFP began to accumulate on meiotic spindle poles and remained on meiotic spindle poles after spindle rotation (T = +60 s).

Picture from Madhu Bhoomi J et al. (2019) MicroPubl Biol

Figure 1. Effects of S. marcescens and S. epidermidis on egg laying and brood size in wild-type and dbl-1(nk3) populations. (A) Comparison of mean eggs laid between wild-type (WT) and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. The counts of number of eggs laid were reported only for plates with live animals. Error bars represent SEM. n = 7-10 surviving animals each. (B) Comparison of total brood size between wild-type and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. Total brood sizes of animals that desiccated were censored. Error bars represent SEM. n = 7-10 surviving animals each. Within each genetic background, significant differences between the E. coli OP50-fed control and the pathogen-fed populations are marked with asterisks (*, p< 0.05, **, p< 0.001).

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Behaviors of plus ends of astral microtubules at the cell cortex. The green box in the schematic embryo indicates the region at the cortex that was magnified. Images of GFP-tubulin and GFP-EB1 were acquired with 0.5 s and 1 s exposure times, respectively. CR, contractile ring. Times (s) are with respect to the onset of posterior movement of the spindle. The scale bar is 5 um.