Picture from Lyssenko NN et al. (2007) Biotechniques "Cognate putative nuclear localization signal effects strong nuclear ...."

Figure 1. NLSEGL-13 enhances the nuclear localization of a GFP gene expression reporter and fa- cilitates recognition of reporter expression sites and identification of reporter-expressing cells. (A) Expression of the NLSSV40-GFP and NLSSV40-GFP-NLSEGL-13 reporters in the pharynx muscle cells. (B) Expression of the NLSSV40-GFP and NLSSV40-GFP-NLSEGL-13 reporters in the hypodermis and seam cells. Arrows, hypodermis nuclei; arrowheads, seam cell nuclei. (C) Expression of the NLSSV40- GFP-NLSEGL-13 reporter in the progeny of the vulval precursor cells and the anchor cell (AC) (left) and in unidentified cells in the head region (right). NLS, nuclear localization signal; EGL-13, egg-laying abnormal-13; GFP, green fluorescent protein; SV40, simian virus 40.

Picture from Zahreddine H et al. (2010) Curr Biol "CRT-1/calreticulin and the E3 ligase EEL-1/HUWE1 control hemidesmosome ...."

Figure 2. Expression Patterns and Fibrous Organelle Phenotypes of Selected evab Genes (A) Conserved domains of EEL-1 and CRT-1. Horizontal black bars indicate extent of deletion alleles ok1575 and ok948; arrow indicates position of GFP insertion. The following abbreviations are used: DUF, domain of unknown function; UBA, ubiquitin-associated domain; HECT, homologousto the E6-AP carboxyl terminus domain. (B) A transcriptional eel-1::gfp construct is expressed at low levels in most cells. (C-J) Immunostaining with NE8/4C6 (muscle; red) and VAB-10A (green) antibodies of vab-10A (e698) (C and H), crt-1(ok948) (D), eel-1(ok1575) (E), vab-10A(e698); crt-1(ok948) (F and I), and vab-10A(e698); eel-1(ok1575) embryos (G and J) at 1.7-fold (H-J) or 3-fold (C-G) stages. Antibodies from boxed areas in (Fa) and (Ga) are enlarged in (Fb) and (Fc) and (Gb) and (Gc), respectively. Arrows in (Fb) and (Gb) indicate areas with detached muscles but normal VAB10A; arrowheads in (Fb) and (Gb) indicate areas with abnormal VAB-10A; dotted lines in (Fc) and (Gc) indicate expected muscle positions; arrowheads in (I) and (J) indicate areas with detached muscles. Scale bar in (C) represents 10 um.