Picture from Maddox AS et al. (2005) Development "Distinct roles for two C. elegans anillins in the gonad and early ...."

Control telophase embryos were fixed and stained for DNA, ANI-1, myosin II (NMY-2) and the septin UNC-59. Images are equivalently scaled. Scale bar: 10 um. (B) Insets from the images in A are magnified twofold. The new cell-cell boundary (black arrows) and contractile ring (C.R., red arrowheads) are indicated. (C) Schematic showing location and orientation of magnified view in D. (D) Embryos undergoing polar body extrusion were fixed and stained for ANI-1, NMY-2 and UNC-59. In control embryos, all three proteins localize to a ring around the site of polar body extrusion. Images have been rotated so that this ring is viewed end on.

Picture from Maddox AS et al. (2005) Development "Distinct roles for two C. elegans anillins in the gonad and early ...."

Figure 3. (A) ANI-1 is required to target the septins, but not myosin II to the contractile ring. Control, ani-1(RNAi), unc-61(e228) and nmy-2(RNAi) telophase embryos were fixed and stained for DNA, ANI-1, myosin II (NMY-2) and the septin UNC-59. Images are equivalently scaled. Only very low amounts of residual ANI-1 are detected in ani-1(RNAi) embryos. The contractile ring at the tip of the ingressing furrow (red arrowheads) and the new cell-cell boundary, which forms behind the ingressing furrow and consists of two juxtaposed cortical surfaces (white arrows), are indicated. The furrow does not ingress in nmy-2(RNAi) embryos. Scale bar: 10 um. (B) Insets from the images in A are magnified twofold. The new cell-cell boundary (black arrows) and contractile ring (C.R., red arrowheads) are indicated. (C) Schematic showing location and orientation of magnified view in D. (D) Embryos undergoing polar body extrusion were fixed and stained for ANI-1, NMY-2 and UNC-59. In control embryos, all three proteins localize to a ring around the site of polar body extrusion. Images have been rotated so that this ring is viewed end on. Scale bar: 10 um.