Expression of PLX-2 in embryonic neuronal and epidermal cells. PLX-2 GFP transgene expression was detected by immunostaining with anti-GFP antibodies (green in all panels) ; samples are labeled with the anti-AJM-1 antibody MH27 (red) to visualize epidermal cell outlines. A and B are stained with DAPI (blue) to show cell nuclei. (A) A PLX-2::GFP translational fusion protein is first expressed in a small number of ventral cells prior to epidermal enclosure; these are identified as ventral neuroblasts on the basis of position. (B) In late stage embryos, PLX-2::GFP was expressed in several tail epidermal cells, including the preanal ventral epidermal cell pairs P11/12 and P9/10 (indicated as 'P' with white lines). PLX-2::GFP was also expressed in numerous neurons in the head and tail. (C and D) Expression of PLX-2 in the QV5 lateral epidermal cell at the 1.5- and 2-fold stage, detected using the Pplx-2-GFP transcriptional reporter ncIs21; images are projections of surface focal planes from a confocal z-stack. Bar, 10 um in all panels except B (20 um).
Figure 6. Expression of PLX-2 and EFN-4 in embryonic neuronal and epidermal cells. PLX-2 and EFN-4 GFP transgene expression was detected by immunostaining with anti-GFP antibodies (green in all panels) ; samples are labeled with the anti-AJM-1 antibody MH27 (red) to visualize epidermal cell outlines. A and B are stained with DAPI (blue) to show cell nuclei. (A) A PLX-2::GFP translational fusion protein is first expressed in a small number of ventral cells prior to epidermal enclosure; these are identified as ventral neuroblasts on the basis of position. (B) In late stage embryos, PLX-2::GFP was expressed in several tail epidermal cells, including the preanal ventral epidermal cell pairs P11/12 and P9/10 (indicated as 'P' with white lines). PLX-2::GFP was also expressed in numerous neurons in the head and tail. (C and D) Expression of PLX-2 in the QV5 lateral epidermal cell at the 1.5- and 2-fold stage, detected using the Pplx-2-GFP transcriptional reporter ncIs21; images are projections of surface focal planes from a confocal z-stack. (E-H) EFN-4::GFP (juIs109) is expressed in a subset of epidermal cells during and after epidermal enclosure. During enclosure (lateral views in E and F; ventral view in G), EFN-4::GFP was detected in the lateral epidermal cells H0 and QV5, in the leading anterior epidermal cells (green's') in the head region, and in the three posterior pairs of P cells (P7/8, 9/10, 11/12) (green P cells marked in AJM-1 channel in F). (H) At the 2-fold stage, EFN-4::GFP is seen in P9/10 and P11/12 (P, ventral view of preanal epidermis); expression also persists in the head ventral epidermis. EFN-4::GFP expression in epidermal cells persists until late embryonic stages and was not detectable in L1 larvae. Bar, 10 um in all panels except B (20 um).
(A-F) Confocal images of UNC-104::mCherry in the dorsal and ventral nerve cord, which are labeled with myrGFP (D-F), in young (A and D) andaged (B, C, E, and F) worms.
(D-F) Wild-type anaphase one-cell stage embryos stained with anti-tubulin (D) and anti-ZYG-8 (F) antibodies. (D)-(F) are at the same magnification. Bar = 10 um. Anti-ZYG-8 antibodies mark the spindle and spindle poles (arrowheads) in wild-type.
(A-F) Expression pattern of the autophagy genes atg-9 (A-C) and atg-2 (D-F) using transcriptional fusions (see Supplemental Experimental Procedures). AIY is identified with cytoplasmic mCh (B and E). (C and F) Mergeimages for (A-B) and (D-E), respectively.
Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).