Picture from Murayama T et al. (2013) Curr Biol "Environmental alkalinity sensing mediated by the transmembrane guanylyl ...."

Figure 2. Chemotaxis Analysis of gcy Mutants and Expression and Ca2+ Imaging of gcy-14 (A) Alkaline pH chemotaxis of mutants defective in gcy genes expressed in ASE (n = 12 assays each). (B) Specific expression of the gcy-14p::gfp reporter construct in ASEL, but not in ASER. Neurons stained with DiI are shown in magenta. ASE cell positions are indicated by arrows. Scale bars represent 10 mm. (C) Subcellular localization of GCY-14::GFP. The arrow indicates the cell body of ASEL. Open and filled arrowheads indicate the distal end of a dendrite and a sensory cilium of ASEL, respectively. The scale bar represents 10 mm. (D) Ca2+ transients in ASEL of gcy-14(pe1102) upon a pH up-step at t = 30 s. Ca2+ transients in ASEL of the wild-type in Figure 1D are also shown as a reference. (E) Ca2+ transients in ASER of wild-type, gcy- 14(pe1102), gcy-14 animals expressing the wildtype gcy-14 gene specifically in ASEL, and unc-13(e450) in response to a pH up-step from 6.8 to 9.1. pH up-steps were at t = 10 s. (F) Mean fluorescence intensity increases in ASER of wild-type and mutant animals shown in (E). Data were calculated from mean values of relative DF/F0 (%) at their peaks. **p < 0.01, Dunnett test compared to the wild-type in (B) and Mann-Whitney U test in (F). NS, not significant. Numbers of recordings are shown in parentheses. Error bars and gray bands in Ca2+ transient traces indicate the SEM. See also Figure S2.

Picture from Murayama T et al. (2013) Curr Biol "Environmental alkalinity sensing mediated by the transmembrane guanylyl ...."

(B) Specific expression of the gcy-14p::gfp reporter construct in ASEL, but not in ASER. Neurons stained with DiI are shown in magenta. ASE cell positions are indicated by arrows. Scale bars represent 10 um.

Picture from Murayama T et al. (2005) J Mol Biol "The dyf-3 gene encodes a novel protein required for sensory cilium formation in ...."

(a), (b) and (d) Expression patterns of functional DYF-3::GFP fusion protein in the wild-type background. (c) Diagram of cell bodies of DYF-3-expressing neurons shown in (a). (d) DYF-3::GFP in cilia of head neurons. The picture shown in (a) was made by merging a fluorescent photograph and a differential interference contrast (DIC) photograph. Scale bars represent 5 um.

Picture from Murayama T et al. (2005) J Mol Biol "The dyf-3 gene encodes a novel protein required for sensory cilium formation in ...."

Figure 6. (a), (b) and (d) Expression patterns of functional DYF-3::GFP fusion protein in the wild-type background. (c) Diagram of cell bodies of DYF-3-expressing neurons shown in (a). (d) DYF-3::GFP in cilia of head neurons. (e) dyf-3::gfp expression in a daf-19(m86) animal. Pictures shown in (a)-(e) were made by merging a fluorescent photograph and a differential interference contrast (DIC) photograph. Scale bars represent 5 um.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of LET-19 in the T-cell division. Expression of let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Figure 5. Symmetric expression of LET-19 and DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) and let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lockhead D et al. (2016) Mol Biol Cell "The tubulin repertoire of C. elegans sensory neurons and its context-dependent ...."

(B) Coexpression of alpha-tubulin and beta-tubulin TagRFP-T protein fusions and uIs31 TRN::GFP transcriptional fusion. Left, uIs31(mec-17p::gfp) showing PLM. Center, TagRFP-T tubulin fusion protein. Right, composite image. TagRFP-T was false colored as magenta for enhanced visibility. Expression of isoforms inTRNs is seen as white in the composite overlap of uIs31 marker and TagRFP-T::Tubulin. Images are oriented so that the anterior of the animal is on the left and the ventral side is on the bottom. Scale bar, 50 um.

Picture from Herman MA (2001) Development "C. elegans POP-1/TCF functions in a canonical Wnt pathway that controls cell ...."

POP-1 is asymmetrically distributed at the T cell division. POP-1 expression in T.a and T.p. Whole-mount larvae 5-7 hours post-hatching, were stained with monoclonal anti-POP-1 antibodies and MH27 monoclonal antibodies, which outline hypodermal cells. The V cell (V) and T cell (T) daughters are indicated. (A) In wild-type animals, the level of POP-1 is higher in Vn.a than in the Vn.p, and also higher in T.a than in T.p. Scale bar: 10 um.