Picture from Mukhopadhyay S et al. (2008) Dev Cell "Sensory signaling-dependent remodeling of olfactory cilia architecture in C. ...."

Figure 1. Sensory Signaling Modulates AWB Cilia Structure. (A and A') Location of the cell body, processes, and cilia of an AWB olfactory neuron in the head of an adult animal. The AWB neuron is visualized via expression of str-1::gfp. Only one member of the bilateral AWB neuron pair is visible in the lateral view shown.(B-F) The cilia of an AWB neuron visualized via str-1p::gfp transgene expression in (B and B') wild-type (WT) adults grown under standard conditions, (C and C') tax-4(ks11) adults, (D and D') odr-1(n1936) adults, (E and E') wild-type adults grown in CeMM, and (F and F') wild-type adults grown in CeMM + bacteria. All cilia images were acquired by using confocal microscopy.(B'-F') represent volumetric reconstructions from confocal projection series. Arrowheads indicate fans. Anterior is oriented toward the left in all images.The scale bar is 7.5 μm for all confocal images.

Picture from Mukhopadhyay S et al. (2008) Dev Cell "Sensory signaling-dependent remodeling of olfactory cilia architecture in C. ...."

Localization pattern of GFP-tagged OSM-6 in the cilia of an AWB neuron.

Picture from Mukhopadhyay S et al. (2008) Dev Cell "Sensory signaling-dependent remodeling of olfactory cilia architecture in C. ...."

Localization pattern of GFP-tagged SRD-23 in the cilia of an AWB neuron.

Picture from Mukhopadhyay S et al. (2008) Dev Cell "Sensory signaling-dependent remodeling of olfactory cilia architecture in C. ...."

(A and A') Location of the cell body, processes, and cilia of an AWB olfactory neuron in the head of an adult animal. The AWB neuron is visualized via expression of str-1::gfp. Only one member of the bilateral AWB neuron pair is visible in the lateral view shown. The cilia of an AWB neuron visualized via str-1p::gfp transgene expression in (B and B') wild-type (WT) adults grown under standard conditions. All cilia images were acquired by using confocal microscopy.(B') represent volumetric reconstructions from confocal projection series. Anterior is oriented toward the left in all images. The scale bar is 7.5 um.

Picture from Mukhopadhyay S et al. (2008) Dev Cell "Sensory signaling-dependent remodeling of olfactory cilia architecture in C. ...."

Figure 3. Localization of Ciliary Proteins in AWB Cilia. (A-E) Shown are the localization patterns of GFP-tagged (A) STR-1, (B) SRD-23, (C) ODR-3, (D) TAX-2, and (E) OSM-6 in the cilia of an AWB neuron in the indicated growth conditions and genetic backgrounds. For klp-11 and bbs-8; odr-1 mutants, the cilia branch length phenotypes exhibited by a subset of these animals are indicated in a second panel in (A). The asterisk in (A) indicates accumulation at the ciliary base in kap-1; osm-3 double mutants. ODR-3::GFP and TAX-2::GFP rescue the corresponding mutant phenotypes, and their localization patterns in these mutant backgrounds are shown. OSM-6::GFP was previously shown to rescue the osm-6 phenotype in the AWB neurons (Mukhopadhyay et al., 2007). Alleles used were odr-1(n1936), tax-4(ks11), egl-19(n2368gf), klp-11(tm324), kap-1(ok676), bbs-8(nx77), osm-3(p802), and grk-2(gk268). WT, wild-type. The scale bars are 15 um.(F) Summary of localization patterns of GFP-tagged proteins in AWB cilia.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)). (C) Overexpression of UNC-33(S) suppresses unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.