Picture from Ishihara T et al. (2002) Cell "HEN-1, a secretory protein with an LDL receptor motif, regulates sensory ...."

Figure 5. Expression of the HEN-1 Protein. (A) The expression of the hen-1::GFP fusion gene in the nervous system of the head region at the L1 stage. Scale bars indicate 10 μm in (A)-(D). Left is anterior and down is ventral.(B) Immunostaining of the wild-type animals with anti-HEN-1-antibody.(C and D) 3D reconstructed images of wild-type (C) and unc-104 (D) animals by immunostaining. The arrowheads indicate punctated staining of the HEN-1 in the nerve ring, whereas the localization of HEN-1 in the axon was diminished in unc-104.

Picture from Ishihara T et al. (2002) Cell "HEN-1, a secretory protein with an LDL receptor motif, regulates sensory ...."

(B) Immunostaining of the wild-type animals with anti-HEN-1-antibody.(C) 3D reconstructed images of wild-type animals by immunostaining. The arrowheads indicate punctated staining of the HEN-1 in the nerve ring.

Picture from Ishihara T et al. (2002) Cell "HEN-1, a secretory protein with an LDL receptor motif, regulates sensory ...."

(A) The expression of the hen-1::GFP fusion gene in the nervous system of the head region at the L1 stage. Scale bars indicate 10 μm in (A)-(D). Left is anterior and down is ventral.

Picture from Hao JC et al. (2010) Dev Cell "The tripartite motif protein MADD-2 functions with the receptor UNC-40 (DCC) in ...."

Figure 4. madd-2::gfp Expression and Preferential Localization of MADD-2 and UNC-40 to the ADL Ventral Branch. (A) Comma stage (bottom) and 1.5-fold stage (top) embryos. The anterior cells that express madd-2::gfp include developing neurons of the nerve ring. Expression is also detected in the ventral and posterior embryo, including motor neurons and hypodermal cells.(B) L1 stage larva. Expression is high in head neurons that project into the nerve ring (arrow). Muscles of the head also express the transgene (arrowhead).(C) L3 stage larva. The HSN motor neuron expresses the madd-2::gfp transgene.(D) An srh-220::gfp transgene uniformly labels both dorsal (black arrowhead) and ventral (arrow) branches of the ADL neuron.(E and F) In both larval (E) and adult (F) animals, the srh-220::madd-2::gfp transgene preferentially labels the ADL ventral branch (arrow).(G) Quantitation of the fluorescence intensity ratio between the ventral and dorsal branches in adult animals expressing GFP, MADD-2::GFP, or UNC-40::GFP in ADL. Asterisks indicate a significant enrichment in ventral branch fluorescence as compared to GFP alone (p < 0.005, t test). Error bars represent the standard error of the mean.(H) An L1 larva expressing srh-220::unc-40::gfp displays preferential expression of UNC-40::GFP in the ADL ventral branch (arrow).For (D)-(F) and (H), the ADL branch point is indicated by a white arrowhead. Anterior is to the left and dorsal is at the top in all panels. Scale bars equal 5 um.

Picture from Linhart C et al. (2012) Genome Res "A novel candidate cis-regulatory motif pair in the promoters of germline and ...."

Expression pattern of additional WT pas-5p::GFP worms from each of the transgenic lines tested (see Figure 3 and Supp. Fig. 4).

Picture from Linhart C et al. (2012) Genome Res "A novel candidate cis-regulatory motif pair in the promoters of germline and ...."

Figure 3. The motif pair in pas-5 promoter is required for germline and somatic gonad expression. (A) Structure of the pas-5p::GFP reporter. 1,245 bp of pas-5 promoter were fused to GFP. The sequence of the wild-type and mutated motifs are shown. (B) A scheme of an adult C. elegans hermaphrodite. The gonad is made of two symmetric U-shaped arms. Each of the arms terminates at the spermatheca. Oocytes are fertilized in the spermatheca, embryos mature in the uterus and laid through the vulva. (C) The germline cells of the gonad. Germ cells proliferate (mitotic zone) in the distal region of the gonad and as they move proximally (transition zone) they enter meiosis and differentiate into sperm or oocyte. (D) The somatic gonad is composed of the distal tip cell (DTC), gonadal sheath, spermatheca (sp), spermathecal-uterine (sp-ut) valve, and uterus. The sheath and DTCs are physically associated with the germline cells and have critical roles in their development and function. The DTCs control gonad migration and regulate entry of the germline cells into mitosis versus meiosis (Kimble and White 1981). The sheath cells are smooth muscle-like cells necessary for oocyte maturation and ovulation (McCarter et al. 1999). (E) Expression pattern of wild-type pas-5p::GFP versus pas-5pM12::GFP reporters in the adult gonad. Expression is detected in the mitotic region of the germline (blue asterisk), the meiotic region (pair of blue asterisks), the DTC (red asterisk and curve) and the gonadal sheath cells (green asterisks). Additional images are shown in Supp. Fig. 6.

Picture from Linhart C et al. (2012) Genome Res "A novel candidate cis-regulatory motif pair in the promoters of germline and ...."

Supp. Fig 5. pas-5p::GFP transgene expressed from an extrachromosomal array. (A) Spermatheca (sp), sheath cells (sh), vulva (vul), uterus (ut), muscle (mu), epidermis (ep). (B) Distal tip cell (dtc).

Picture from Linhart C et al. (2012) Genome Res "A novel candidate cis-regulatory motif pair in the promoters of germline and ...."

Supp. Fig 6. Expression pattern of additional WT pas-5p::GFP and pas-5pM12::GFP worms from each of the transgenic lines tested (see Figure 3 and Supp. Fig. 4). The gonad distal tip cells (DTCs) in the mutant transgenes are marked with red arrows.

Picture from Hao JC et al. (2010) Dev Cell "The tripartite motif protein MADD-2 functions with the receptor UNC-40 (DCC) in ...."

(A) Comma stage (bottom) and 1.5-fold stage (top) embryos. The anterior cells that express madd-2::gfp include developing neurons of the nerve ring. Expression is also detected in the ventral and posterior embryo, including motor neurons and hypodermal cells.(B) L1 stage larva. Expression is high in head neurons that project into the nerve ring (arrow). Muscles of the head also express the transgene (arrowhead).(C) L3 stage larva. The HSN motor neuron expresses the madd-2::gfp transgene. Scale bars equal 5 um.

Picture from Shibata Y et al. (2000) Genetics "EAT-20, a novel transmembrane protein with EGF motifs, is required for efficient ...."

Figure 4.-EAT-20 expression in nonpharyngeal tissues of wild-type animals. Bars, 10 um. (A) An 88-cell embryo. The surfaces of all the cells are stained. (B) A comma stage embryo. Arrows indicate the digestive tube. An arrowhead indicates an extrapharyngeal cell. (C) The same embryo as in B at a different focal plane. An arrow indicates the tip of a labial process bundle. Arrow- heads indicate the coelomocytes. (D and E) Seam cells in an L3 larva. Seam cells are stained with the anti-EAT-20 polyclonal antibody (D) and co-stained with the monoclonal antibody MH27 to show the cell boundary (E; Hresko et al. 1994). Asterisks indicate seam cells. (F) Lateral view of an L1 larva with the anterior side to the right and the ventral side up. Arrows indicate the motor neurons in the ventral cord. A row of seam cells is also stained below. (G) Ventral view of an adult animal showing the staining of the ventral hypo- dermal ridge and the vulval hypodermis. Arrows indicate the position of the ventral nerve cord in the ventral hypodermal ridge. (H) Lateral view of the tail of an adult hermaphrodite. The anal hypodermis (arrowheads) and a segment of the ALN axon (arrow) are stained. (I) Lateral view of an adult male tail. Sensory rays are stained. (J) Anterior end of a head in an adult. An arrow indicates an unidentified cell posterior to the tip of labial process bundles.