Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Behaviors of plus ends of astral microtubules at the cell cortex. The green box in the schematic embryo indicates the region at the cortex that was magnified. Images of GFP-tubulin and GFP-EB1 were acquired with 0.5 s and 1 s exposure times, respectively. CR, contractile ring. Times (s) are with respect to the onset of posterior movement of the spindle. The scale bar is 5 um.

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

(A) Time-lapse images of GFP-tubulin, GFP-EB1, GFP-moe, and GFP-RHO-1 at the cortex in live embryos. Arrowheads indicate accumulation of GFP-moe or GFP-RHO-1 at the equatorial cortex. Arrows indicate cleavage furrow formation. Times (s) are with respect to the onset of posterior movement of mitotic spindles at metaphase. In all panels, anterior is oriented toward the left. The scale bar is 10 um.

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Figure 2. Behaviors and Distribution of Astral Microtubules at the Cell Cortex of Wild-Type Embryos. (A) Behaviors of plus ends of astral microtubules at the cell cortex. Magnified view of the time-lapse image series of GFP-tubulin (left) and GFP-EB1 (right) shown in Figure 1. The green box in the schematic embryo indicates the region at the cortex that was magnified. Images of GFP-tubulin and GFP-EB1 were acquired with 0.5 s and 1 s exposure times, respectively. CR, contractile ring. Times (s) are with respect to the onset of posterior movement of the spindle. The scale bar is 5 μm.(B) Time-line quantification of the number and length of GFP-EB1 comets at the cortex. Images were acquired with 1 s or 0.2 s exposure times. All GFP-EB1 comets per embryos in a single cortical focal plane were scored. The data are the means of results from ten independent embryos. Times (s) are with respect to the onset of posterior movement of the spindle.(C) Temporal change of density of microtubule ends at the cortex during mitosis. (Top) Kymographs showing the location of microtubules over time were created from the time-lapse images of GFP-tubulin in Figure 1A for the regions shown outlined in green in the schematic embryo. A representative kymograph set from four independent recordings is shown. Similar results were obtained from the other recordings. Blue line: onset of ingression at the cortical plane. (Bottom) Time points of events before and during cytokinesis. Filled circles: distance between spindle poles as measured by GFP-tubulin. Contractile ring formation was determined by using GFP-moe signals; each black diamond represents the time point of contractile ring assembly in a single embryo.(D) Cortical distribution of GFP-EB1 along the anterior-posterior axis during contractile ring assembly (left) and at the onset of furrowing (right). The cortical area was divided into six regions, as shown by the green lines in the schematic embryo, and the density of GFP-EB1 comets in each area is shown. DP, division plane. The data are the means ± SD of ten independent experiments. Times (s) are with respect to the onset of posterior movement of the spindle.

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Figure 1. GFP Markers for Astral Microtubules and the Contractile Ring. (A) Time-lapse images of GFP-tubulin, GFP-EB1, GFP-moe, and GFP-RHO-1 at the cortex in live embryos. Arrowheads indicate accumulation of GFP-moe or GFP-RHO-1 at the equatorial cortex. Arrows indicate cleavage furrow formation. Times (s) are with respect to the onset of posterior movement of mitotic spindles at metaphase. In all panels, anterior is oriented toward the left. The scale bar is 10 um.(B) Contractile ring assembly is dependent on microtubules and RHO-1. (Top) Accumulation of GFP-moe at the equator was dependent on filamentous actin, RHO-1, and microtubules. No GFP-moe signal was observed in latrunculin-A (Lat-A)-treated embryos or rho-1(RNAi) embryos. The cortical ring was disrupted in the tba-2(RNAi) embryos. (Bottom) Accumulation of GFP-RHO-1 at the equator requires LET-21/RhoGEF and microtubules. In tba-2(RNAi) embryos, both GFP-moe and GFP-RHO-1 were dispersed over the cortex. Arrowheads indicate accumulation of GFP-moe or GFP-RHO-1 at the equatorial cortex. The scale bar is 10 um.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of Rap1GAP (F) as determined by promoter-GFP reporter constructs. (F) Expression of Rap1GAP in hypodermal cells (hd).

Picture from Li LB et al. (2016) Curr Biol "The Neuronal Kinesin UNC-104/KIF1A Is a Key Regulator of Synaptic Aging and ...."

(A-F) Confocal images of UNC-104::mCherry in the dorsal and ventral nerve cord, which are labeled with myrGFP (D-F), in young (A and D) andaged (B, C, E, and F) worms.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(d-f) ptp-2::gfp produced fluorescence in some head neurons (d), gut and spermatheca (e), and body wall muscle (f).

Picture from David DC et al. (2010) PLoS Biol "Widespread protein aggregation as an inherent part of aging in C. ...."

Pkin-19::kin-19::tagrfp animals (F and G).

Picture from Gonczy P et al. (2001) Dev Cell "zyg-8, a gene required for spindle positioning in C. elegans, encodes a ...."

(D-F) Wild-type anaphase one-cell stage embryos stained with anti-tubulin (D) and anti-ZYG-8 (F) antibodies. (D)-(F) are at the same magnification. Bar = 10 um. Anti-ZYG-8 antibodies mark the spindle and spindle poles (arrowheads) in wild-type.

Picture from Stavoe AK et al. (2016) Dev Cell "KIF1A/UNC-104 Transports ATG-9 to Regulate Neurodevelopment and Autophagy at ...."

(A-F) Expression pattern of the autophagy genes atg-9 (A-C) and atg-2 (D-F) using transcriptional fusions (see Supplemental Experimental Procedures). AIY is identified with cytoplasmic mCh (B and E). (C and F) Mergeimages for (A-B) and (D-E), respectively.