Picture from Moore LL et al. (1999) J Cell Biol "HCP-1, a protein involved in chromosome segregation, is localized to the ...."

Figure 5. HCP-1 is localized to the centromere/kinetochore. Embryos were fixed in cold methanol and stained with mAb 6C4 and either MPM-2 or anti-HCP-3 antibody as described in Materials and Methods. Chromosomes were visualized by staining with DAPI. Images were collected using the Deltavision microscopy and analyzed as stacks. (A) A one-cell embryo in metaphase is shown stained with MPM-2 antibody (red) and DAPI (blue). The MPM-2 antibody primarily stained the poleward face of metaphase chromosomes. (B) The same embryo stained with mAb 6C4 (green) and DAPI (blue). (C) The two channels for MPM-2 (red) and mAb 6C4 (green) staining were merged using Photoshop (Adobe Systems Inc.) to identify the colocalization (yellow). D and E show a late prophase nucleus stained with mAb 6C4 (green), anti-HCP-3 (red), and DAPI (blue). F shows the two channels for anti-HCP-3 (red) and mAb 6C4 (green) staining merged (yellow). G depicts a single prophase chromosome (arrow in F) enlarged fourfold and rotated 90 degrees into the plane of the paper (H) to show the specific localization to only opposite surfaces of the chromosome. Bar, 2 um.

Picture from Moore LL et al. (1999) J Cell Biol "HCP-1, a protein involved in chromosome segregation, is localized to the ...."

Figure 2. Dynamic localization of mAb 6C4 antigen throughout the cell cycle. Two-cell C. elegans embryos were fixed and stained with mAb 6C4 (A, D, G, J, and M), DAPI (B, E, H, K, and N). C, F, I, L, and O show the merged mAb 6C4 (red) and DAPI (blue) staining. Coverslips were imaged on Deltavision microscope and 0.2-um optical sections were obtained as described in Materials and Methods. The images were deconvolved and analyzed as a projection of the entire stack. Nuclei at different positions in the cell cycle are shown: A-C, early prophase; D-F, late prophase; G-I, metaphase; J-L, anaphase; and M-O, metaphase/anaphase I of meiosis. Bar, 5 um.

Picture from Moore LL et al. (1999) J Cell Biol "HCP-1, a protein involved in chromosome segregation, is localized to the ...."

Figure 1. mAb 6C4 stains the poleward face of C. elegans metaphase chromosomes. A 28-cell C. elegans embryo was fixed and stained according to methods described in Materials and Methods with mAb 6C4 (red in A and C) and DAPI (blue in B and C). Nuclei in metaphase (m), prophase (p), and late anaphase/telophase (t) are indicated. Bar, 10 um.

Picture from Moore LL et al. (2001) J Cell Biol "HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for ...."

Figure 3. Centromeric association of HCP-4 during mitosis. Two-cell C. elegans embryos at different stages of the mitotic cell cycle were fixed and stained with anti-HCP3 antibody (A, D, G, J, and M, green), anti-HCP4 (B, E, H, K, and N, red), and DAPI (blue). Colocalization of HCP-3 and HCP-4 was visualized in the merged images as yellow (C, F, I, L, and O). All images, except metaphase, are a projection of the Z-stack of the entire nucleus. (A-C) Early prophase; (D-F) late prophase; (G-I) metaphase; (J-L) anaphase; (M-O) telophase. Bars, 5 um.

Picture from Moore LL et al. (2001) J Cell Biol "HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for ...."

HCP-4 localization in C. elegans embryos. (A) Western blot of wild-type embryo extract (60 ug total protein) probed with anti-HCP-4 antibody (diluted 1:500) shows that a single band of 136 kD is present. All other bands, including the band at 70 kD (*), were independent of the primary antibody. Wild-type embryos were stained with DAPI (B), anti-HCP-3 antibody (C), and anti-HCP-4 antibody (D). Approximately equal staged embryos containing both mitotic (M) and interphase cells are shown. Exposure times were normalized relative to the HCP-3 staining. Arrow indicates prophase nucleus with nuclear staining. Bar, 5 um.

Picture from Moore LL et al. (2001) J Cell Biol "HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for ...."

Figure 2. HCP-4 localization in C. elegans embryos. (A) Western blot of wild-type embryo extract (60 ug total protein) probed with anti-HCP-4 antibody (diluted 1:500) shows that a single band of 136 kD is present. All other bands, including the band at 70 kD (*), were independent of the primary antibody. Wild-type embryos or hcp-4(RNAi) embryos were stained with DAPI (B and E), anti-HCP-3 antibody (C and F), and anti-HCP-4 antibody (D and G). Approximately equal staged embryos containing both mitotic (M) and interphase cells are shown. Exposure times were normalized relative to the HCP-3 staining. Arrow indicates prophase nucleus with nuclear staining. Bar, 5 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.