Picture from Lai CC et al. (1996) J Cell Biol "Sequence and transmembrane topology of MEC-4, an ion channel subunit required ...."

Figure4. MEC-4 is expressed in the six touch receptor neurons and precluded from the nucleus. (A) Immunoreactivity in a whole mount preparation of strain ZB2 incubated with anti-NH2-terminal MEC-4 antibody AbM4(1-69). ZB2 harbors an integrated ar- ray of a mec-4 lacZ fusion gene (Mitani et al., 1993). Indicated are the six touch receptor neurons. This photograph is a composite of three photographs taken to view different touch cells in the same focal plane. (B) Detail of anti-MEC-4 immunoreactivity in the two tail touch receptors, PLML and PLMR. (C) DAPI-stained nuclei in the tail of the animal depicted in B. (Arrows) Touch receptor nuclei. Overlay of photographs in B and C indicates that the dark inner area typically seen in stained cells corresponds to the nucleus.

Picture from Kwan CS et al. (2008) BMC Physiol "TRPM channels are required for rhythmicity in the ultradian defecation rhythm of ...."

Supplementary figure 2. The C. elegans TRPM channels gtl-1 and gtl-2 are expressed in the intestine and pharynx and excretory cell respectively.A-E: gtl-1 is expressed in the intestine. The intestine is marked by white arrows in panels A-D and a black arrow in panel E. Panels A-D show transgenic animals carrying GFP fused to part of the gtl-1 gene created as below. A, expression in the intestine (white arrows) of an adult. B, a closer view of the intestine (white arrow) of a young adult. C an oblique section in which GFP expression in the lining of the intestine (white arrow) is clear. D, expression in the intestines (white arrows) of two three-fold embryos. E, In situ hybridization of gtl-1 in an L1 larva, F,G: gtl-2 is expressed in the pharynx and excretory cell. A fusion of gtl-2 and GFP was created ina similar way to gtl-1::GFP above (see below). F, expression in the pharynx GFP is observed throughout the muscle cells, parts of the excretory cell are also visible(arrowhead). G, expression in the excretory cell (white arrow) of an L2 larva. The canals are labeled. The animal shown in G did not show expression in the pharynx and was used for clarity. GFP fusions for gtl-1 were constructed as follows. The 5' end of the gtl-1 mRNA was determined by 5' RACE (unpublished). A PCR fragment extending from 4.8 kb upstream of the ATG start codon to the start of exon 2 was subcloned upstream of GFP in the vector pHAB200 [1] so that the gtl-1 and GFP sequences were translationally fused. Similarly a PCR fragment extending from exon 30 to 2kb downstream of the stop codon of gtl-1 was translationally fused downstream of GFP.The final plasmid, called pCK5 was microinjected into C. elegans [2] at 20 μgμl-1together with a rol-6 marker [3] and PvuII digested C. elegans genomic DNA (75μg μl^-1). Stable transformants were selected and analyzed using a Leica SP1 Confocal Microscope. Three independent lines were analyzed. GFP fusions for gtl-2 were created in a similar way. The upstream fragment extended from 5.4 kb upstream of the ATG to the middle of exon 3. The downstream fragment extended from exon 25to 2.4 kb downstream. The final plasmid (pCK7) was injected into C. elegans at 20μg μl^-1 with the rol-6 marker. In situ hybridization was carried out using the method of Mitani et al., [4] and Ogawa et al., [5].