Picture from Thompson-Peer KL et al. (2012) Neuron "HBL-1 patterns synaptic remodeling in C. elegans."

(A and B) A representative image and summary data of hbl-1 (HgfpH, green) and GAD (red) reporter expression in wild-type L3 animals. Yellow arrows indicate DD cell bodies expressing both markers, white arrows indicate DD cell bodies lacking HgfpH expression, and carrots indicate VD cell bodies. HgfpH expression was significantly lower in VD than DD neurons (p < 105 by Kolmogorov-Smirnov test; 150 DD (black) and 260 VD (gray) cells were analyzed).

Picture from Meng J et al. (2017) Dev Cell "Myrf ER-Bound Transcription Factors Drive C.elegans Synaptic Plasticity via ...."

(E) Co-localization of native GFP signal from GFP::MYRF-1 (ybqEx164) and unc-25pro-mCherry::RAB-3 (juIs236). Square box, area with closer view of DD to theright; rectangular box, area with closer view of DD/VD to the right; dotted circles, DD or VD soma. Scale bar, 10 um.

Picture from Wang Q et al. (2002) J Neurosci "The C domain of netrin UNC-6 silences calcium/calmodulin-dependent protein ...."

Figure 4. npr-1 is expressed in DD and VD ventral nerve cord motor neurons. A, B, Immunofluorescence micrographs of a larva expressing a npr-1:: gfp reporter. The larva was stained with anti-GFP antibodies (A) and anti-GABA antibodies (B). NPR-1:: GFP is coincident with the GABAergic motor neurons DD and VD of the ventral nerve cord (arrowheads). Scale bar, 25 um.

Picture from Petersen SC et al. (2011) J Neurosci "A transcriptional program promotes remodeling of GABAergic synapses in ...."

Figure 4. irx-1::GFP is expressed in remodeling GABAergic motor neurons. A, IRX-1::GFP is restricted to the nucleus of DD motor neurons in wild-type L4 larvae. In unc-55 mutants, IRX-1::GFP is observed in both DD and VD motor neurons (DD5 and VD10 shown for examples). Similar expression patterns were observed for all DD and VD motor neurons. Scale bar, 5 um. B, Quantification of IRX-1::GFP expression in GABAergic motor neurons of L2 larvae. Note that IRX-1::GFP is de-repressed in unc-55 VD motor neurons. ***p< 0.0001; NS, not significant; Fisher's exact two-tailed test.

Picture from Demarco RS et al. (2010) PLoS Genet "RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in Caenorhabditis ...."

Figure 5. RACK-1::GFP expression in GABAergic motor neurons. Micrographs are of animals harboring an unc-25::rack-1::gfp transgene that expresses a full-length RACK-1::GFP fusion protein specifically in the VD/DD GABAergic motor neurons. A) RACK-1::GFP expression is specific to the VD/DD motor neurons. Arrowheads indicate cell bodies in the ventral nerve cord, and arrows indicate commissural axons. The punctate fluorescence throughout the animal is autofluorescence of the gut. B) RACK-1::GFP accumulated in a VD growth cone in an early L2 animal (arrow). The diagram at the right traces the outline of the growth cone and axon. The scale bars represent 5 um.

Picture from Thompson-Peer KL et al. (2012) Neuron "HBL-1 patterns synaptic remodeling in C. elegans."

Figure 2. UNC-55 COUP-TF Represses hbl-1 Expression in VD Neurons (A and B) A representative image and summary data of hbl-1 (HgfpH, green) and GAD (red) reporter expression in wild-type L3 animals. Yellow arrows indicate DD cell bodies expressing both markers, white arrows indicate DD cell bodies lacking HgfpH expression, and carrots indicate VD cell bodies. HgfpH expression was significantly lower in VD than DD neurons (p < 105 by Kolmogorov-Smirnov test; 150 DD (black) and 260 VD (gray) cells were analyzed). (C-F) HgfpC (C and D) or HmutgfpC (E) transcriptional reporter expression (green) is compared for adjacent VD10 and DD5 neurons in wild-type (C and E) and unc-55 (D) mutant animals. Gray lines connect VD10 and DD5 cells in the same animal, black lines connect median values (pvalues by paired Student's t test). Average log2 of the ratio of DD5 to VD10 fluorescence for HgfpC and HmutgfpC was plotted in (F), and n = number of animals analyzed (*p < 105 difference from WT; ns, p = 0.2; by Student's t test). Error bars indicate SEM. See also Figure S2.

Picture from Tilmann C et al. (2005) Dev Cell "Cyclin D regulation of a sexually dimorphic asymmetric cell division."

Figure 2. q626 Feminizes the Gonad and Appearsto Function Like fkh-6 (A-C) L3 animals carrying anchor cell (AC) marker, cdh-3::GFP. (A) Wild-type hermaphrodite. (B) Wild-type male. (C) q626 male raised at the restrictive temperature expresses the AC marker. (D-F) L4 animals carrying marker for vas deferens (VD) and seminal vesicle (SV),K09C8.2::GFP. (D) Wild-type hermaphrodite. (E) Wild-type male. (F) q626 male raised at the restrictive temperature does not expressthe SV/VD marker. (G-I) L1 gonads viewed by Nomarski microscopy. Asterisks, germ cells. (G) Male SGP daughters differ in size. Small, open arrow, smaller distal daughter; large, open arrow, larger proximal daughter. (H) Hermaphrodite SGP daughters are of roughly equal size. White arrowhead, distal daughter; black arrowhead, proximal daughter. (I) q626 SGP daughters at the restrictive temperature are of roughly equal size. Same conventions as (H).

Picture from Jin YS et al. (1999) J Neurosci "The Caenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase and ...."

Figure 4. The expression pattern ofunc-25. A, An adult worm of genotype lin-15(n765); juEx[pSC100+lin-15(EK)]. GFP is expressed in all and in only GABAergic neurons. RMEs, DVB, AVL, and RIS are marked by arrows; arrowheads point to several DD and VD neurons. Not all DD and VD neurons are seen as a consequence of the mosaic expression of the transgene.B, An adult worm of genotype unc-25(e156); lin-15(n765); juEx[pSC317+ plin-15(EK)]. UNC-25:: GFP is found in cell bodies (arrowheads), axons, and commissures (arrow). C, The ventral cord of an adult worm of genotype unc-25(e156); lin-15(n765); juEx[pSC317+ plin-15(EK)]. UNC-25:: GFP is associated with synaptic varicosities seen as punctate fluorescent clusters (arrows). Arrowheads mark neuronal cell bodies. D, The ventral cord of an adult worm of genotype unc-104(e1265); lin-15(n765); juEx[pSC317+ plin-15(EK)]. No punctate synaptic fluorescent clusters are visible. The arrowhead marks a neuronal cell body. Scale bar: A, 50 um; B-D, 170 um.

Picture from Ashley, Nicholas et al. (2019) MicroPubl Biol

Figure 1 Homozygous bec-1(ok691) mutants show a delay in the development of VD motor neurons. (A-C) Representative fluorescent micrographs of D-type motor neuron expressing GFP in (A) control, (B) homozygous bec-1(ok691) mutant, and (C) heterozygous bec-1(ok691) mutant nematodes on day 5 of adulthood. (D) Quantification of D-type motor neuron cell bodies on days 0,1,3,5, and 7 of adulthood was recorded. Data plotted are mean ± 1 SEM, n=60. Statistical analysis was performed using a non-parametric Kruskal-Wallis (p< .001).

Picture from Ashley, Nicholas et al. (2019) MicroPubl Biol

Homozygous bec-1 (ok691) mutants show a delay in the development of VD motor neurons. (A) Schematic of bec-1 gene shown in light blue and bec-1 (ok691) deletion mutation in yellow (wormbase.org). (B) D-type motor neurons marked by GFP in homozygous bec-1 (ok691) mutant nematodes on day 5 of adulthood. (C) Quantification of D-type motor neuron cell bodies on days 0,1,3,5,and 7 of adulthood was recorded. Data plotted are mean ± 1 SEM, n=60. Statistical analysis was performed using a non-parametric Kruskal-Wallis (p< .001).