Figure 4. MI Fate Specification Is Sensitive to Histone H3 Mutations (A) Expression of translational
his-9(+)::mStrawberry (top panel) and
his-9(H113D)::mStrawberry (bottom panel) genes during embryonic (left panel) and larval (right panel) stages. Nomarski images (middle panel) of the same larvae and percentages of animals with the MI transformation defect are shown. Arrows indicate the MI neuron, and arrowheads indicate the
e3D and the
e3D-like epithelial cells. Scale bar, 5 mm. (B) The genomic positions of
his-9 and 13 additional replication-dependent histone H3 genes that encode proteins with amino acid sequences identical to that of HIS-9 are shown. Numbers represent approximate genetic map positions. Percentages of animals with the MI transformation defect are shown.