Picture from Melendez A et al. (2003) Science "Autophagy genes are essential for dauer development and life-span extension in ...."

(A) GFP::LGG-1 expression in the seam cells of wild-type N2 and daf-2(e1370) L3 larvae grown at 15C and 25C. The arrow shows representative GFP-positive punctate areas that label preautophagosomal and autophagosomal structures. (B) Quantitation of GFP::LGG-1-positive punctate areas in the seam cells of wild-type N2 and daf-2(e1370) animals grown at 15C and 25C (mean + SEM for at least 50 seam cells from 10 different animals).

Picture from Melendez A et al. (2003) Science "Autophagy genes are essential for dauer development and life-span extension in ...."

Figure 4. Dauer formation in daf-2(e1370) mutant animals is associated with an increase in autophagy in lateral hypodermal seam cells. (A) GFP::LGG-1 expression in the seam cells of wild-type N2 and daf-2(e1370) L3 larvae grown at 15C and 25C. The arrow shows representative GFP-positive punctate areas that label preautophagosomal and autophagosomal structures. (B) Quantitation of GFP::LGG-1-positive punctate areas in the seam cells of wild-type N2 and daf-2(e1370) animals grown at 15C and 25C (mean + SEM for at least 50 seam cells from 10 different animals). (C to I) Representative electron micrographs of lateral hypodermal seam cells in a wild-type N2 control animal in (C) the L2 larval stage and (D) the L3 larval stage; a daf-2(e1370) animal grown for (E) 1 day at 25C and [(F) to (H)] 2 days at 25C; and (I) a daf-2(e1370); bec-1 (RNAi) animal grown for 2 days at 25C. In (F), arrowheads denote representative vacuolar structures in the late stages of the autophagolysosomal pathway; the white arrowhead denotes structure that is shown at higher magnification in (H); the arrow denotes the dauer cuticle. (G) A representative early autophagosome and (H) a multilamellar body/autolysosome are shown in a seam cell of a daf-2(e1370) dauer animal. Scale bars, 1 uM [(C) to (F) and (I)]; 0.1 uM [(G) and (H)].

Picture from Melendez A et al. (2000) Genetics "Caenorhabditis elegans lin-13, a member of the LIN-35 Rb class of genes involved ...."

Figure 3. Analysis of lin-13::lacZ and LIN-13::GFP. (A-C) lin-13::lacZ expression in hyp7 in L3 larvae. Larvae were fixed and stained with an anti-LacZ antibody (red) as well as DAPI to visualize nuclei. (A) Anti-LacZ; (B) DAPI; (C) merged anti-LacZ antibody (red) and DAPI. (D and E) Nuclear localization of LIN-13::GFP. (D) Living embryo. Fluorescence is nuclear. Note also the discrete fluorescent foci within the nuclei. (E) Lateral hypodermal seam cells in an L3 larva. Larvae were fixed and stained with an anti-GFP antibody to visualize LIN-13::GFP (red). Larvae were also stained with MH27, a monoclonal antibody that detects adherens junctions (green; Priess and Hirsh 1986).

Picture from Toth ML et al. (2007) J Cell Sci "Influence of autophagy genes on ion-channel-dependent neuronal degeneration ...."

Figure 4. BEC-1 and LGG-1 are expressed in the nervous system. (A) A non-functional translational fusion BEC-1::GFP reporter accumulates in the PVM of a wild-type animal. Both cell body and processes (arrowhead) of this cell are GFP positive. VNC, ventral nerve cord. (B) BEC-1 expression in the nerve ring (bracket). (C) LGG-1::GFP, which lacks the C terminus of LGG-1 (see Materials and Methods), is expressed in PLML. (D) LGG-1::GFP accumulation in the ventral nerve cord. (E) CeTor, which encodes a kinase that downregulates autophagy, is expressed in all neurons. Bracket indicates the nerve ring. (F-H) Nomarski, fluorescence and merged images, respectively, showing the expression of a non-integrated, translational fusion (functional) autophagosome marker GFP::LGG-1 (Melendez et al., 2003) in a PVC interneuron of a deg-3(u662) animal. The GFP-positive punctate areas are inferred to label preautophagosomal and autophagosomal structures (Melendez et al., 2003). In this specimen, the PVC interneuron is at the early stage of degeneration. Note that GFP becomes gradually diluted as degeneration progresses. The nearly exclusive expression of GFP in PVC is due to a mosaicism of the non-integrated marker.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.