Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

(B) RUND-1 localizes to the Golgi. Left: a backscatter scanning electron micrograph of the cell body of a neuron in the nerve ring. Right: the same image overlaidwith the corresponding fluorescence PALM image of RUND-1::tdEos. Scale bar, 1 mm.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

Figure 5. RUND-1 Colocalizes with RAB-2 at the trans-Golgi Network(A) RUND-1 colocalizes with RAB-2 and trans-Golgi markers. Each panel shows a single slice of a confocal image of motor neuron cell bodies in the ventral cord of young adult animals. The top boxes show the localization of a single-copy rescuing RUND-1::tagRFP-T fusion protein (oxIs590). RUND-1 localizes almost exclusively to two or three perinuclear puncta per cell. The middle boxes show the localization of single-copy GFP-tagged compartment markers. The bottom boxes show the merged images. Scale bar, 5 μm, applies to all panels. RUND-1 shows tightest colocalization with RAB-6.2, a trans-Golgi Rab protein, and with SYX-6, the ortholog of the trans-Golgi SNARE syntaxin 6. RUND-1 puncta also colocalize well with RAB-2 puncta. RUND-1 partially overlaps with the medial Golgi marker mannosidase II (AMAN-2) and the cis-Golgi marker µCOP. RUND-1 is not colocalized with the rough ER marker TRAM-1 or with the endosomal markers RAB-5, RAB-11.1, RAB-7, and SYN-13.(B) RUND-1 localizes to the Golgi. Left: a backscatter scanning electron micrograph of the cell body of a neuron in the nerve ring. Right: the same image overlaidwith the corresponding fluorescence PALM image of RUND-1::tdEos. Scale bar, 1 μm.(C) CCCP-1 colocalizes with RUND-1 and RAB-2. CCCP-1 colocalizes with RUND-1 and RAB-2(GTP). CCCP-1 is still punctate when coexpressed with RAB-2(GDP), which is diffusely expressed.(D) The RUN domain of RUND-1 mediates its localization. Full-length RUND-1, the coiled-coil domain, and the RUN domain were tagged at their C termini with tagRFP-T and integrated in the genome. The truncated proteins were expressed at lower levels. See also Figure S8.