Picture from McGee MD et al. (2009) J Cell Sci "KDP-1 is a nuclear envelope KASH protein required for cell-cycle ...."

GFP::KDP-1 localizes to the NE. (A) Single focal planes of fixed embryos or gonads expressing GFP::KDP-1 were probed with anti-GFP (first column and green in merge) and anti-lamin (second column and red in merge) antibodies and stained with DAPI to observe chromatin (third column and blue in merge). Images of an embryo are in the first row, pachytene regions of gonads in row 2, and maturing oocytes in the proximal gonad in row 3. Scale bar: 10 um. (B) Single focal plane of a GFP::KDP-1 embryo probed with the GFP (green in merge) and KDP-1 (red in merge) antibodies and stained with DAPI (blue in merge). Scale bar: 10 um. (C) Live larvae expressing GFP::KDP-1 in various tissues including (i) the ventral cord, (ii) hypodermal cells, (iii) body wall muscles, (iv) neurons and pharynx. NE enrichment is visible in the ventral cord and hypodermal cells. Scale bar: 10 um.

Picture from McGee MD et al. (2009) J Cell Sci "KDP-1 is a nuclear envelope KASH protein required for cell-cycle ...."

Figure 3. GFP::KDP-1 localizes to the NE. (A) Single focal planes of fixed embryos or gonads expressing GFP::KDP-1 were probed with anti-GFP (first column and green in merge) and anti-lamin (second column and red in merge) antibodies and stained with DAPI to observe chromatin (third column and blue in merge). Images of an embryo are in the first row, pachytene regions of gonads in rows 2-3, and maturing oocytes in the proximal gonad in rows 4-5. Samples in rows 3 and 5 were from sun-1(RNAi) animals. Scale bar: 10 um. (B) Single focal plane of a GFP::KDP-1 embryo probed with the GFP (green in merge) and KDP-1 (red in merge) antibodies and stained with DAPI (blue in merge). Scale bar: 10 um. (C) Live larvae expressing GFP::KDP-1 in various tissues including (i) the ventral cord, (ii) hypodermal cells, (iii) body wall muscles, (iv) neurons and pharynx. NE enrichment is visible in the ventral cord and hypodermal cells. Scale bar: 10 um.

Picture from McGee MD et al. (2009) J Cell Sci "KDP-1 is a nuclear envelope KASH protein required for cell-cycle ...."

Figure 2. Antibodies against KDP-1 localize to the NE of germline nuclei. (A) Western blot of worm lysates from wild-type (N2) or transgenic (TG) worms expressing GFP::KDP-1. Blot was probed with pre-immune serum (left) or the affinity-purified KDP-1 antibody (right). Size in kDa is shown on the right. (B) Dissected adult hermaphrodite gonad stained with DAPI to show nuclei. The mitotic zone is the most distal part of the gonad (DG). Stages of meiotic prophase I are marked in orange. Individual bivalents can be seen in diakinesis oocytes at the proximal end of the gonad (PG). DTC is the region covered by the distal tip cell and PCD is the region of the gonad where most programmed cell death occurs. Image is a composite of three gonads; dashed lines correspond to intervening areas not covered by individual gonad images. Adapted from (Lints and Hall, 2005). (C) Single focal plane of fixed wild-type germline nuclei from the mitotic zone through early pachytene probed with anti-KDP-1 (row 1 and red in merge) and anti-nuclear pore complex (row 2 and green in merge) antibodies and stained with DAPI (row 3 and blue in merge). Scale bar: 10 um. Box represents zoomed region to the right.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).