Picture from McDiarmid, Troy A et al. (2020) MicroPubl Biol

Figure 1. Lifelong Auxin exposure does not affect a suite of phenotypes scored by the Multi-Worm Tracker in N2 and PD1074 wild-type C. elegans strains: The Auxin-Inducible Degradation (AID) system relies on genetically tagging a protein of interest (POI) with an AID degron peptide tag and transgenic expression of the inducible E3 ubiquitin ligase TIR1 (A). The presence of Auxin triggers TIR1 to polyubiquitinate the POI, causing subsequent protein degradation (A). N2 and PD1074 animals were age synchronized onto standard Nematode Growth Media (NGM) plates or 0.025mM or 1mM Auxin plates (B). Animals were subjected to a short-term habituation behavioral paradigm and (C) the Multi-Worm Tracker (MWT) was used to quantify the effects of Auxin on morphological and behavioral phenotypes. For N2 and PD1074 animals, Auxin exposure at either 0.025mM or 1mM did not affect initial response sensitivity or habituation of response probability, speed, or duration (D and E), or 5 commonly studied morphological (animal length and width) and locomotion (movement speed, directional bias, and degree of kink in body posture (related to curvature of body posture)) phenotypes (F and G). A detailed description of all phenotypes can be found in the MWT user guide (Swierczek et al. 2011).