(A) The muscarinic receptors
gar-2 and
gar-3 are not required for suppression of
acr-2(gf) by arecoline. (B) Pre-treatment with the specific muscarinic antagonist atropine does not affect suppression of
acr-2(gf) by arecoline. (C) Loss of function in genes involved in GABAergic synaptic transmission eliminates suppression of
acr-2(gf) by arecoline. (D) A new
unc-49(
ju1617) mutation was isolated in a genetic screen for mutants preventing suppression of
acr-2(gf) by arecoline. Two-way ANOVA, ***P<0.001, **P<0.01, *P<0.05, n.s.=not significant.