unc-71 expression pattern. (F,G) UNC-71::GFP may be localized to the cell membrane. Punc-115UNC-71::GFP expression is seen in the nerve ring (arrow in F) and along the nerve cords(arrow in G). (H,I) Pe15*2UNC-71::GFP expression in hypodermis is seen near the nerve cords (arrow in H,I). Arrowhead in H indicates the vulva hypodermis. *Likely GFP aggregates. Scale bars: 20 um in F-I.
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.
Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.
(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).
(A) and (C): DIC images of the embryos shown in (B) and (D). E-H. At postembryonic stages, epg-11::gfp is expressed in pharyngeal cells (E,F, arrows) and vulval cells (G,H, arrow). (E) and (G): DIC images of the animals shown in (F) and (H).