Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Spatial miRNA promoter activity. (Top) DIC images; (bottom) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Figure 5. miRNAs from a given family can have overlapping as well as different spatiotemporal expression patterns. (A) Cartoon depicting expression patterns of 10 complete and two incomplete (let-7 and mir-80 families shown at the bottom) miRNA families. Each color represents a family. Spa- tiotemporal expression is as in Supplemental Table S3. (B) miRNAs from the mir-35 family are expressed in overlapping tissues/cell types. Pmir35-41::gfp and Pmir-42-44::gfp are shown. (C) miRNAs from the mir-75 family are expressed in different tissues/cell types. (Top) DIC images; (bottom) GFP fluorescence. (D) Definition and example of TsOC between any two miRNAs. (E) Distribution of TsOC among miRNA pairs from the same or different family.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Spatial miRNA promoter activity. (Top) DIC images; (bottom) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Pmir-82 drives expression in pharyngeal muscle and head neurons (left), and developing spermatheca (right). (Top) DIC images; (bottom) GFP fluorescence. Arrows indicate expression. Dotted arrows indicate sequence used as promoter.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Spatial miRNA promoter activity. (Top) DIC images; (bottom) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Spatial miRNA promoter activity. (Top) DIC images; (bottom) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Temporal miRNA promoter activity. Representative images of miRNA promoters that drive GFP expression in the embryo. (Left) DIC images; (right) GFP fluorescence.