Figure 1. Validation of a chromosome II single-copy
eft-3p::TIR1::F2A::BFP::AID*::NLS transgene:
eft-3p::TIR1::F2A::BFP::AID*::NLS animals (late L3 stage) were shifted onto control or 4 mM K-NAA (1-Naphthaleneacetic Acid, Potassium Salt) auxin plates for the indicated times (0, 30, 60, 120 minutes). (A) Animals were collected and DIC, BFP, and GFP imaging of the head region was performed. Scale bars represent 10 µm. (B) Western blots detecting AID*::GFP in control- or auxin (4 mM K-NAA)-treated animals. Anti-GFP blots (top) detected background bands (marked with *) also reported by Ashley et al., 2021 and a doublet at approximately 27 kDa, consistent with the predicted size of GFP. Total protein is provided as a loading control. Equal exposure times were used to collect each set of GFP and BFP images.