Picture from Bhattacharya R et al. (2009) J Cell Sci "The PAPS transporter PST-1 is required for heparan sulfation and is essential for ...."

Figure 6. Subcellular localization of PST-1 and PST-2. (A) Schematic of C-terminal fusions of PST-1A, PST-2 and of a mannosidase II fragment (MANS) that targets expression to the Golgi (Rolls et al., 2002) with the fluorescent proteins are indicated. All constructs are under control of the pst-1a promoter (supplementary material Fig. S7). Not drawn to scale. (B) PST-1A::YFP and PST-2::CFP display partial colocalization. Magnification, 630x. (C) The Golgi marker MANS::RFP displays partial colocalization with both PST-1A::YFP and PST-2::CFP. (D) Triple stain of PST-1A::YFP, PST-2::CFP and the MANS::RFP Golgi marker. Arrowheads denote lack of colocalization in an intestinal cell in all cases. All insets created with 2x digital zoom. Magnification, 630x.

Picture from Chen CC et al. (2006) Mol Biol Cell "RAB-10 is required for endocytic recycling in the Caenorhabditis elegans ...."

Figure 7. RFP-RAB-10 partially colocalizes with a Golgi marker (MANS-GFP) in the intestine. Images of the TOP focal plane (A-C) show basolateral membranes and cortex, whereas images of the MIDDLE focal plane (D-F) show the intestine in cross-section. Arrowheads indicate overlapping signals generated by MANS-GFP and RFP-RAB-10. Arrows indicate puncta positive for RFP-RAB-10 only. The asterisk (*) indicates the lateral plasma membrane and 'L' indicates the position of apical lumen of the intestine. Bar, 10 um.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 5. RAB-6.2 associates with Golgi and GLR-1. (A and A') Cerulean:: RAB-6.2 (A) and MANS::YFP (A') in PVC cell bodies. (A'') Merged image showing Cerulean::RAB-6.2 colocalization to puncta with MANS::YFP (arrowheads). (B-C'') GLR-1::CFP (B and C) and Venus::RAB-6.2 (B' and C') in PVC cell bodies (B-B'') and dendrites (C-C''). (B'' and C'') Merged images show colocalization at specific points (arrowheads). (D-F) Subcellular localization of the indicated GFP::RAB-6.2 variant: wild type (D), GDP-locked mutant (E), and GTP-locked mutant (F). Bars, 5 um.

Picture from Prasad BC et al. (2006) Development "Wnt signaling establishes anteroposterior neuronal polarity and requires ...."

VPS-35 expression and localization. (C) vps-35::gfp; GFP expression in embryo. (D) vps-35::gfp; GFP expression in tail epidermis of a young larva. (E) Golgi marker mannosidase-YFP (MANS-YFP) expression in tail epidermis using the lin-44 promoter. (F) VPS-35-mCherry expression in tail epidermis. (G) Merged image of E and F. A-D, anterior is leftwards; E-G, anterior is rightwards; dorsal is towards the top.

Picture from Luo L et al. (2011) Mol Biol Cell "The Caenorhabditis elegans GARP complex contains the conserved Vps51 subunit ...."

Figure 3: GARP complex localizes to the Golgi endosomal interface. (A) GARP complex localization overlaps with the medial Golgi (Mans::GFP) and late Golgi (GFP::GRIP domain) and partially with endosomal domains (GFP::2xFYVE) in body-wall muscles. Insets show magnifications of the marked regions of the merged fluorescence images. Scale bar, 5 μm. (B) Thin plastic section EM picture of a Golgi ministack present in C. elegans body-wall muscles. Scale bar, 200 nm. (C) GARP complex localization overlaps with the medial Golgi (Mans::GFP) but not with endosomal domains (2xFYVE::GFP) in coelomocytes. Scale bar, 5 μm. (D) Thin plastic section EM picture of Golgi stacks present in C. elegans coelomocytes. Scale bar, 500 nm. (E) VPS-52::mCherry can be colocalized with the C. elegans Rab6 orthologues GFP::RAB-6.1 and GFP::RAB-6.2. (F) VPS-52 can be coimmunoprecipitated with both Rab6 orthologues; GFP::RAB-10 was used as a negative control. Scale bar, 5 μm.

Picture from Arellano-Carbajal F et al. (2011) PLoS Genet "Macoilin, a conserved nervous system-specific ER membrane protein that ...."

Figure S6 MACO-1 is an ER resident protein. Confocal optical sections showing neuronal cell bodies in worms bearing extrachromosomal arrays that express either the YFP-tagged nuclear envelope marker emerin (A-D), or the YFP-tagged Golgi marker mannosidase (E-H), and co-stained with anti-MACO-1 antibodies (B, F), anti-GFP antibodies (A, E) and DAPI (C, G). Arrowheads indicate co-localisation between the MACO-1 and the nuclear envelop marker. EMR, Emerin; MANS, Mannosidase. Scale bars represent 1 micron.

Picture from Miyara A et al. (2011) PLoS Genet "Novel and conserved protein macoilin is required for diverse neuronal functions ...."

Figure 3. Subcellular localization of MACO-1. (A, B) Subcellular localization of the almost full-length genomic maco-1::GFP, including predicted transmembrane domains and coiled coil domains in wild-type animals. (A) Merged differential interference contrast (DIC) and green fluorescent protein (GFP) images. (B) GFP image. Anterior is to the left and dorsal is up. (C-N) Co-expression of MACO-1 or human ortholog of MACO-1 (FLJ10747) fused to yellow fluorescent protein (YFP) or cyan fluorescent protein (CFP) with organelle markers in AFD thermosensory neurons of wild-type animal. The following markers were used: CFP-tagged translocating chain-associating membrane protein (CFP::TRAM) as a marker of rough endoplasmic reticulum (rER); CFP-tagged mannosidase (MANS::CFP) as a marker of Golgi bodies; membrane anchored YFP (YFP::GPI) as a marker of plasma membranes [29]. (C-E) Co-expression of YFP::MACO-1 with CFP::TRAM. (F-H) Co-expression of YFP::FLJ10747 with CFP::TRAM. (I-K) Co-expression of YFP::MACO-1 with MANS::CFP. (L-N) Co-expression of YFP::GPI with CFP::MACO-1. Scale bars: A and B = 5 um; C-N = 2 um.

Picture from Treusch S et al. (2004) Proc Natl Acad Sci U S A "Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 ...."

Figure 3. Morphological characterization of large vacuoles in coelomocytes of cup-5(ar465) worms. Shown are confocal images of WT or of cup-5(ar465) coelomocytes expressing the indicated GFP fusions (green) 24 h after BSA-Rhod (red) injection into the body cavity. Large arrows indicate large vacuoles, small arrows indicate lysosomes, arrowheads indicate late endosomes, the asterisk indicates heterogeneity in BSA-Rhod staining within the large vacuoles, and the small compartments stained by mannosidase II::GFP (MAN::GFP) are indicated with a G. Coelomocytes are outlined. (Bar, 5 um in all images.)

Picture from Darom A et al. (2010) Mol Biol Cell "RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in ...."

Expression pattern of RNF-121. (B and C) RNF-121::GFP is localized to the ER in body wall muscles (mu), hypodermis (hyp), and seam cells (SE). Bar, 10 um. (D) RNF-121::GFP colocalizes with the mRFP::SP12 reporter. Bar, 5 um. (E and F) RNF-121::GFP is expressed in the somatic gonad and vulva. Lateral view of midL4 animal. Expression is detected in the vulval cells (vul), spermathecal cells (sp), uterine cells (ut), and the distal tip cell (DTC). Bar, 10 um. (G) RNF-121::GFP colocalizes with the mRFP::mans reporter. Bar, 5 um.

Picture from Prasad BC et al. (2006) Development "Wnt signaling establishes anteroposterior neuronal polarity and requires ...."

Figure 6. VPS-35 expression and localization. (A) Wild-type mab-5::gfp; GFP expression is seen in QL descendants QL.a and QL.p in L1. (B) vps-26; mab-5::gfp expression is not detected in QL descendants. Positions of cells were determined by DIC microscopy (denoted by arrows). (C) vps-35::gfp; GFP expression in embryo. (D) vps-35::gfp; GFP expression in tail epidermis of a young larva. (E) Golgi marker mannosidase-YFP (MANS-YFP) expression in tail epidermis using the lin-44 promoter. (F) VPS-35-mCherry expression in tail epidermis. (G) Merged image of E and F. A-D, anterior is leftwards; E-G, anterior is rightwards; dorsal is towards the top.