Fig 4. GFP::MAI-1 is diffusely expressed in the cytoplasm and some nuclei. We mounted one-day-oldadult animals expressing the transgene Pmai-1::gfp::
mai-1-3'UTR and observed them under a confocalmicroscope. We observed the expression of GFP::MAI-1 in the cuticle (A-B), cytoplasm and nuclei (arrow) ofthe hypodermis (C-D), in the rectum, vulva, and coelomocytes (E) and in some neurons of the animal's head(E,F). Details of each picture are shown in the right (inset). We stained GFP::MAI-1 animals with MitoTrackerCMXRos and observed no co-localization between them (G-I). Details of each picture are shown on thebottom (J-L). h: head, t: tail, c: cuticle, hyp: hypodermis, rect: rectum, coelomocytes: cc.