Picture from Kage-Nakadai E et al. (2011) Biochem Biophys Res Commun "H+/myo-inositol transporter genes, hmit-1.1 and hmit-1.2, have roles in the ...."

Fig. 1. Expression of the HMIT family in C. elegans. (A) Expression patterns of transcriptional translational fusion genes. Photographed animals were young adult hermaphrodites. Phmit-1.1::egfp is expressed in the intestine (left panel). Phmit-1.2::egfp is expressed in the amphid sheath glia as indicated by the arrow and the excretory canal cell as indicated by the arrowhead (middle panel). Phmit-1.3::egfp is expressed in the amphid sheath glia as indicated by the arrow and in the intestine as indicated by the arrowhead (right panel). Scale bar = 100 lm. (B) HMIT-1.1::EGFP is localized at the lumenal membrane of the intestine. Arrowheads indicate the lumenal membrane and arrows indicate the basal membrane. A young adult hermaphrodite of a transgenic rescue line tm3301(hmit-1.1);tmEx2407[Phmit-1.1::hmit-1.1::egfp] was photographed. Scale bar = 20 lm. (C) HMIT-1.2::EGFP is localized in the glial cell body (arrow) and the endfoot region (arrowhead), in which cilia of sensory neurons are embedded. The imaged animal was a young adult hermaphrodite of a transgenic rescue line, tm3196(hmit-1.2);tmEx2408[Phmit-1.2::hmit-1.2::egfp]. Scale bar = 50 lm. (D and E) HMIT-1.2::EGFP is localized at the lumenal membrane of the proximal canal (D) and the basal membrane of the distal canal (E). This localization pattern was exhibited in all the analyzed young adult hermaphrodites of a transgenic rescue line tm3196;tmEx2732[Phmit-1.2::hmit-1.2::egfp, Phmit-1.2::DsRed] (n = 5). DsRed fluorescence completely fills the space between the canal lumen (arrowheads) and the basal membrane (arrows). The asterisk indicates the excretory canal cell body. Scale bar = 10 um. (F) Schematic drawing of the tissue distribution and subcellular localization of HMIT family in C. elegans.

Picture from Weinberg F et al. (2014) Gene Expr Patterns "Expression pattern and first functional characterization of riok-1 in ...."

Fig. 4. Expression pattern of riok-1 during vulval development in the larval stages of the riok-1 reporter line: (A-F) DIC images; (A0 - F0) GFP images; (A00 - F00) merged images. (A and B): In the L2-larva, GFP expression was observed in the somatic gonad as well as in the ventral nerve cord. (C): In the L3-larva, riok-1 expression is maintained in the somatic gonad in ventral uterine (VU/AC) cells. (D): riok-1 is expressed in the committed anchor cell (arrow). Note that riok-1 is not detectable in the VPCs in the hypodermis. (E and F): In the L4-larva, riok-1 is expressed in the vulF, the utse and cells of the vulva and uterus. Scale bars 10 lm; (A-D), (F) lateral views, (E) ventral view.

Picture from Ziel JW et al. (2009) Gene Expr Patterns "An expression screen for RhoGEF genes involved in C. elegans ...."

Fig. 1. (A-D) Schematic representation of larval gonad development in C. elegans. (A) After hatching, during the L1 stage, the somatic gonad precursor cells Z1 and Z4 surround the primordial germ cells and are located on the anterior and posterior sides of the early gonad. (B) During the L2 stage these cells give rise to somatic lineages of the gonad including the distal tip cells (DTCs) and the cells of the somatic gonad primordium. (C) During the L3 stage the cells of the somatic gonad primordium divide to form the dorsal and ventral uterine cells (DU/VU cells) as well as the gonad sheath and spermathecal cells. (D) During the L4 stage, the uterine and spermathecal epithelia form mature lumens. The DTCs and the gonad sheath cells complete their migrations. (E-H) DIC micrographs overlaid with LAM-1::GFP fluorescence labeling the basement membrane which surrounds and outlines the gonad. RhoGEF 50 CRE activity was scored in: (E) Z1 and Z4 at the L1 stage; (F) the somatic gonad primordium at the L2 stage; (G) the AC, VU/ DU cells, the spermathecal and sheath lineage and the DTCs during the L3 stage and (F) the uterine and spermathecal epithelia at the L4 stage. (I-L) Selected examples of RhoGEF 50CRE activity during gonadogenesis: (I) 50CREs from C28C12.10 were active in Z1 and Z4; (J) C02F12.4 was detected in the somatic gonad primordium; (K) T19E10.1/ ect-2, K07D4.7b, F55C7.7a,b/unc-73 and C11D9.1 50CRE activity detected in the DU/VU cells, AC, DTC and spermathecal and sheath lineages respectively; (L) K11E4.4/pix-1 50 CREs were active broadly within the uterine epithelium, whereas elements from C11D9.1 were active specifically within the spermatheca. Scale bar in (E) represents 20 lm. Abbreviations: epithelium, Ep; distal tip cell, DTC; anchor cell, AC; dorsal uterine cell, DU; ventral uterine cell, VU in this and all subsequent figures.