Picture from Ma L et al. (2012) PLoS Genet "The Caenorhabditis elegans gene mfap-1 encodes a nuclear protein that affects ...."

Figure 2. MFAP-1 is a nuclear protein expressed in multiple tissues. (A) A 2.5 kb 5' promoter region upstream of the start codon of mfap-1 was used to drive the expression of a transcriptional fusion GFP reporter in transgenic animals. The tissues in which the GFP reporter was expressed are labeled. This figure shows a montage of two photographs, which were pseudo-colored from a gray-scale image to a green-scale image using Adobe Photoshop to reflect the level of GFP expression. (B) Transgene experiments were performed as described in Materials and Methods. The C. elegans MFAP-1::GFP fusion protein, when expressed in body-wall muscles using a myo-3 promoter in either wild-type or mfap-1(n4564 n5214) animals of mixed developmental stages, was exclusively localized in nuclei (left and middle panels). Similarly, human MFAP-1::GFP fusion protein, when expressed in the body-wall muscles of mfap-1(n4564 n5214) animals, was also exclusively localized in nuclei (right panels). Lower panels show typical nuclear GFP signals in adult transgenic animals. Genetic backgrounds and transgenes are indicated.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(L) Pgrl-6::GFP is expressed in the PVT neuron.

Picture from Munro E et al. (2004) Dev Cell "Cortical flows powered by asymmetrical contraction transport PAR proteins to ...."

(J-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-3.

Picture from Yin J et al. (2017) J Cell Biol "GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in ...."

(J-L") Confocal fluorescence images in wild type coexpressing GFP::GOP-1and markers of different organelles including Cherry::RAB-5 (J-J", early endosome), Cherry::RAB-7 (K-K", late endosome), or NUC-1::Cherry (L-L", lysosome). White arrowheads, vesicles with overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in closeproximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right (mean +- SD). Bars: (A-I') 10 um; (J-L") 5 um.

Picture from Tian Y et al. (2010) Cell "C. elegans screen identifies autophagy genes specific to multicellular ...."

(G-L) The epg-4 reporter is expressed ubiquitously in embryos (H) and widely in larvae (I). EPG-4::GFP colocalizes with the ER marker cherry::TRAM-1 (J-L). (G) Nomarski image of the embryo in H.

Picture from Pujol N et al. (2001) J Biol Chem "The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ...."

In situ hybridization images on embryonic, larval, and adult worms with vha-6 (I-L) are shown. vha-6 is expressed only in the intestine, from the embryonic coma stage (I) through larvae (J and K) and adult (L).

Picture from Mahoney TR et al. (2008) Proc Natl Acad Sci U S A "Intestinal signaling to GABAergic neurons regulates a rhythmic behavior in ...."

(G'-L') aex-2 is expressed in the GABAergic neurons AVL and DVB as well as in enteric muscles. (G and I) AEX-2::mCherry is detected in the nerve ring (NR, arrow), ciliary sensory processes (CA, open arrow), nerve cord (I), and head mesodermal cell (HMC, arrowhead). (H) AEX-2::mCherry is expressed in the intestinal muscle (IM, arrow) and anal depressor (AD, arrowhead). (J'-L') mCherry expressed under the aex-2 promoter is detected in AVL (J-L, arrow) and DVB (J''-L'', arrow) GABAergic neurons. AEX-2::mCherry signal does not significantly overlap with GABAergic GFP in the ventral nerve cord (J'-L'). (Scale bar, 20 um.) See SI Text for transgenes and clones.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Expression pattern of ptr-10::myrRFP reporter transgene. (G-L) Merged fluorescence and DIC images (G,I,K) and fluorescence images (H,J,L) of an adult animal expressing ptr-10::myrRFP. Expression in the head (G,H), vulva (I,J) and rectum (K,L) is shown. Arrowheads in H indicate the expression in sheath and socket glia of IL, OL (blue), and OLQ (yellow) sensilla, as well as in CEPsh glia (white).

Picture from Liu ZC et al. (1995) Development "The Caenorhabditis elegans heterochronic gene pathway controls ...."

Wild-type larvae (L) and adult (A) hermaphrodites transformed with the col-19/lacZ fusion. Bar 100 μm.

Picture from Dorfman MD et al. (2009) Genetics "Using RNA interference to identify specific modifiers of a ...."

Spinning-disk confocal projected Z-stack images of tdTomato fusion proteins driven by the tdTomato::RFL-1 promoter. (J-L) tdTomato::RFL-1. (J) Tail neurons and intestine in adults. (K) Vulval VulB2 cells in adult. (L) Larval head. Anterior is to the left. Bars, 10 um.