Picture from Luo J et al. (2015) Genetics "Neuropeptide receptors NPR-1 and NPR-2 regulate Caenorhabditis elegans ...."

Figure 4 npr-2 is expressed in multiple neurons and functions in the AIZ interneurons to regulate the MeSa avoidance response. (A) npr-2 gene structure (designed using the Exon-Intron Graphic Maker software at www.wormweb.org based on gene sequence information at www.wormbase.org). The ok419 mutation deletes a region including exon 5 (partial), exons 6, 7, and 8, and exon 9 (partial) of npr-2 isoform a. (B) MeSa avoidance response of npr-2(ok419) mutants expressing an npr-2:: GFP transgene under control of an endogenous npr-2 promoter. Statistics: different from wild type. Error bars: standard errors. *P , 0.01 (Bonferroni correction after one-way ANOVA). (C) A GFP transgene under control of the endogenous npr-2 promoter is expressed in multiple sensory neurons and interneurons. (D) MeSa avoidance responses of npr-2 (ok419) mutants expressing an npr-2::GFP transgene under control of different neuron-specific promoters. Target neurons are indicated. Three lines were assayed for each transgene. Statistics: different from npr-2 (ok419). Error bars: standard errors. *P , 0.05 (Bonferroni correction after one-way ANOVA).

Picture from Luo J et al. (2015) Genetics "Neuropeptide receptors NPR-1 and NPR-2 regulate Caenorhabditis elegans ...."

(C) A GFP transgene under control of the endogenous npr-2 promoter is expressed in multiple sensory neurons and interneurons.

Picture from Luo J et al. (2015) Genetics "Neuropeptide receptors NPR-1 and NPR-2 regulate Caenorhabditis elegans ...."

Figure S2 npr-2 is expressed in sensory neurons and interneurons.(A) An npr-2p::GFP transgenic animal in low (i, ii) and high magnifications (iii- vi) showing GFP-positive neurons (green) and DiI-labeled neurons (light red). ASH is labeled by GFP and DiI. The highly branched dendrites of PVD and FLP sensory neurons are shown in v and vi. A: anterior; P: posterior; D: dorsal; V: ventral.(B) Penetrance of npr-2p::GFP transgene expression in neurons. 16 animals were scored. O: GFP-positive; X: GFPnegative.

Picture from Luo J et al. (2009) Genetics "The Caenorhabditis elegans ing-3 gene regulates ionizing radiation-induced ...."

Figure 3. Immunolocalization of ING-3. (A) Mouse polyclonal anti-ING-3 recognized endogenous ING-3 as a single 45-kDa band on Western blots of wild-type gravid hermaphrodites. This band was absent in the two ing-3 mutants but was present in cep-1 lysates. Actin was used as the loading control. (B) ING-3 colocalized with chromatin in the newly fertilized embryo (top left) but is absent from ing-3(tm2530) (top right). ING-3 was also found on chromatin at mitosis in developing embryos (arrows, bottom row). (C) Immunostaining of dissected wild-type adult gonads (left-most column) shows that ING-3 overlapped DAPI. Lower levels were present in the mitotic region (I) compared to the transition zone (II). High ING-3 persisted into the early pachytene region (III), but then decreased as cells proceeded through meiosis. Low levels, mainly on chromosomes, were seen at diplotene (IV). Higher magnification of the early pachytene section of the gonad (columns 2-4) showed extensive overlap between ING-3 and DAPI. The nuclear signal disappeared when antisera was preincubated with the immunizing peptide (peptide block) or in ing-3(tm2530). Thus the low level of ING-3 staining between cells likely represents background. The right-most column shows the staining pattern for ING-3 in the anterior nuclei of a dissected gut.

Picture from Luo J et al. (2009) Genetics "The Caenorhabditis elegans ing-3 gene regulates ionizing radiation-induced ...."

Figure 2. Reporters driven by the operon or internal promoter give different expression patterns. (A) The 5' genomic sequence (600 bp) of the first gene of this operon (Y51H1A.3) was used as the 'operon promoter,' while the 880 bp between ing-3 and Y51H1A.3 was used as the 'internal promoter.' (B) GFP and (C) LacZ indicate that the operon promoter was widely expressed in L1 larva, especially in the pharynx. (D) The operon promoter showed GFP expression in the adult vulva, spermatheca (asterisks), neurons, and epidermal cells, among others. (E) Differential interference contrast image corresponding to D. The internal promoter expressed GFP (F) and LacZ (G) in the L1 intestine, the limits of which are indicated by asterisks. The signal between the asterisks in F represents gut auto-fluorescence. Note higher expression in the anterior intestinal cells.

Picture from Inoue T et al. (2004) Proc Natl Acad Sci U S A "Type II platelet-activating factor-acetylhydrolase is essential for ...."

(I and J) paf-2::GFP expression in embryos (lateral view, dorsal is up). Nomarski micrographs (I) and corresponding paf-2::GFP expression (J). paf-2 reporter gene constructs are expressed in epidermal cells (J, arrowheads) and intestinal cells (J, arrow) at the beginning of elongation. Anterior is to the left. (Bar, 10 um.)

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.

Picture from Zhu Z et al. (2016) Dev Cell "Functional Coordination of WAVE and WASP in C.elegans Neuroblast ...."

(J-L) Fluorescence images (J) and quantification(K and L) of ARX-2 (GFP knockin). (J) Upper,merged image; middle, plasma membrane andhistone; lower, ARX-2. Arrows, leading edges;asterisks, ARX-2::GFP from an adjacent apoptoticcell. Box plots show the quantification of ARX2::GFP enrichment

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

I and J, nhx-3:: GFP expression in hypodermal cells (hyp).