Figure 5.
cam-1 regulates RMED/V neurite A/P guidance. (A) RME neuron fluorescence images of animals with different genetic backgrounds. The
cam-1 partial loss-of-function mutant
xd13 shows a variable phenotype and the null mutant
gm122 has a
cwn-2 like phenotype. (B) Molecular lesions in the
cam-1 mutants
xd22,
gm122,
gm105,
ks52 and
xd13. The color-coded domain structure of
cam-1 is shown. Ig, immunoglobulin domain; CRD, cysteine rich domain; Kr, kringle domain; TM, transmembrane domain; Kinase, kinase domain; S/T, serine and theronine-rich domain. Three cam- 1 isoforms are indicated. (C) Quantification of the rescuing activity of
cam-1 transgenes. (D) Quantification of the RMEV neurite A/P outgrowth defect in different mutants.
cam-1 functions synergistically with
cfz-2 and
mig-1. The average relative neurite length in wild type is set as 100. Error bars represent SEM. (E)
cam-1 is expressed in RME neurons (arrow).