Picture from Chen WI et al. (1996) J Biol Chem "The Caenorhabditis elegans p21-activated kinase (CePAK) colocalizes with ...."

Figure 6. Expression of the CePAK mRNA during development. A, blot containing total RNA from six C. elegans developmental stages (E, L1 to L4, and A) was subjected to Northern blot analysis. The autoradiogram was exposed at 70 C for 4 days. B, the same filter was stripped and probed with a 32P-labeled actin cDNA (23). The autoradiogram was exposed at 70 C overnight. C, signals from autoradiograms were quantified separately by scanning densitometry in an Ultroscan XL laser densitometer (Pharmacia Biotech Inc.). The data are normalized to the integrated intensities of signals of C. elegans actin mRNA obtained at each developmental stage. The relative abundance (%) of both mRNAs at each stage is shown.

Picture from Chen WI et al. (1993) J Biol Chem "A new member of the ras superfamily, the rac1 homologue from Caenorhabditis ...."

FIG.3. Developmental regulation of the expression of the CErac1 mRNA. a, totalRNA was prepared from the various C. elegant developmental stages and samples (5pg) from embryos(E), larvae(L1 to L4), adults (A), and mature adults(MA)were subjected to Northern blot analysis as described under 'Experimental Procedures.' The autoradiogram was exposed a t -70 C for 3 days. b, the same filter was stripped in 50% formamide solution, then hybridized with a 32P-labeled cDNA probe for actin mRNA (18),and the auto- radiogram was exposed over night a t -70 C. c, signals from autoradiograms were quantified separately by scanning densitometry in a n Ultroscan XL laser densitometer. The data were normalized to the integrated intensities of signals obtained a t each developmental stage with a 32P-labeled cDNA probe for actin mRNA. The relative abundance of the 1.7-kb CEracI mRNA at each developmental stage is shown.

Picture from Phillips CM et al. (2005) Cell "HIM-8 binds to the X chromosome pairing center and mediates chromosome-specific ...."

The HIM-8 Protein Localizes to the PC Region of the X Chromosome. All images show projections through fields of pachytene-region nuclei from animals of the indicated genotypes. The entire nuclear volume is shown in each projection. All scale bars represent 5 μm.(A) Wild-type hermaphrodite, revealing a single HIM-8 focus in each nucleus due to close association between the two binding sites detected in premeiotic and very early meiotic nuclei (Figure 3).(B) Wild-type male, which displays a single (unpaired) HIM-8 focus in each nucleus. In contrast to hermaphrodites, males also have one HIM-8 focus per nucleus in the premeiotic region of the gonad (data not shown). (E) HIM-8 immunostaining was performed in conjunction with FISH to two probes on the X chromosome, one from the left arm (1.5 Mb from the telomere) and one from a more medial position (7.4 Mb from the left end). The XL probe is closely associated with HIM-8 focus, while the XC probe is clearly more distant from the HIM-8 focus. Probes to the right end of the X and autosomal loci were also tested (data not shown) and did not correlate in their position with HIM-8 foci.

Picture from Yan X et al. (2006) J Gen Physiol "Function of a STIM1 homologue in C. elegans: evidence that store-operated Ca2+ ...."

Figure 2. STIM-1::GFP expression pattern. (A) Low magnification confocal image of a whole worm. STIM-1::GFP expression is present throughout the intestine, but is more pronounced in the anterior (AI) and posterior intestine (PI). Expression is also present in the spermatheca (Sp) and gonadal sheath cells (SC). Bar, 100 um. (B) Expression of STIM-1::GFP in head neurons. Arrows and arrowheads point to cell bodies and dendrites, respectively. Bar, 20 um. (C) Series of confocal Z-sections through the anterior intestine. STIM-1::GFP appears to be localized to a membrane or submembrane region as well as punctate and presumably intracellular domains. Lu, intestinal lumen. Bar, 20 um. (D) Series of confocal Z-sections through the posterior intestine. STIM-1::GFP appears to be localized to a membrane and/or submembrane region and a prominent intracellular reticular structure that resembles the intestinal endoplasmic reticulum (Rolls et al., 2002). Bar, 10 um. (E) Series of confocal Z-sections through the spermatheca (Sp) and gonadal sheath cells (SC). Punctate localization pattern of STIM-1::GFP can be seen in sheath cells in the second and third panels. Bar, 10 um.

Picture from Labbe J-C et al. (1999) Genetics "The levels of the RoRNP-associated Y RNA are dependent upon the presence of ROP-1, ...."

Figure 4. CeY RNA is transcribed at higher levels in the adult germline and in embryos. Total RNA was prepared from staged worms and 5 ug of total RNA was resolved by formalde- hyde-agarose gel electrophoresis, transferred, and probed with (A) a rop-1 cDNA, (B) the yrn-1 gene, or (C) a portion of the gene encoding actin. The levels of rop-1 mRNA and CeY RNA are both high in the embryos, but decrease at the subsequent larval stages. However, the levels of CeY RNA increase a little at the L4 stage and a lot at the adult stage. Lane 1, wild-type embryos; lane 2, wild-type L1 larvae; lane 3, wild-type L2 larvae; lane 4, wild-type L3 larvae; lane 5, wild-type L4 larvae; lane 6, wild-type nongravid adults; lane 7, glp-1(q231) mutant adults. (D) The intensity of each band obtained in (B) was deter- mined by scanning the X-ray film with a LKB ultroscan XL enhanced laser densitometer (Pharmacia, Piscataway, NJ). Each individual band in (B) was corrected with the densitomet- ric value obtained by scanning the band in the corresponding lane obtained in (C) with an actin probe. The corrected data were plotted on a graphical chart.

Picture from Phillips CM et al. (2005) Cell "HIM-8 binds to the X chromosome pairing center and mediates chromosome-specific ...."

Figure 4. The HIM-8 Protein Localizes to the PC Region of the X Chromosome. All images show projections through fields of pachytene-region nuclei from animals of the indicated genotypes. The entire nuclear volume is shown in each projection. All scale bars represent 5 μm.(A) Wild-type hermaphrodite, revealing a single HIM-8 focus in each nucleus due to close association between the two binding sites detected in premeiotic and very early meiotic nuclei (Figure 3).(B) Wild-type male, which displays a single (unpaired) HIM-8 focus in each nucleus. In contrast to hermaphrodites, males also have one HIM-8 focus per nucleus in the premeiotic region of the gonad (data not shown).(C) Hermaphrodite carrying two copies of the mnDp66 duplication of the X chromosome PC region. An additional HIM-8 focus is present in each nucleus, corresponding to the paired and synapsed duplication.(D) Hermaphrodite carrying both mnDp66 and the PC deficiency meDf2, which eliminates one of the two foci observed in (C).(E) HIM-8 immunostaining was performed in conjunction with FISH to two probes on the X chromosome, one from the left arm (1.5 Mb from the telomere) and one from a more medial position (7.4 Mb from the left end). The XL probe is closely associated with HIM-8 focus, while the XC probe is clearly more distant from the HIM-8 focus. Probes to the right end of the X and autosomal loci were also tested (data not shown) and did not correlate in their position with HIM-8 foci.

Picture from Lee RC et al. (2001) Science "An extensive class of small RNAs in Caenorhabditis elegans."

Figure 1. Northern blots of small RNA transcripts. (A through C) Total RNA from C. elegans larvae (stages L1 through L4) or from mixed stage (M) populations were blotted and probed with oligonucleotides complementary to either the 5' or 3' half of the indicated transcript (13). U6 = the same filters were probed with probe to U6 snRNA as a loading control. (A)mir-60 5' probe detects a transcript of ~65 nt. The ratio of L1 to L4 mir-60 signal, normalized to U6, is about 5:1. The mir-60 3' probe (not shown) detects a similar-sized species with a similar developmental profile. (B) mir-80 3' probe detects a ~22-nt RNA expressed uniformly at all stages. (C) mir-52 5' probe. The normalized mir-52 signal is threefold greater in the L1 versus the L3. (D) mir-1 3' probe detects a transcript of ~22 nt in total RNA from mouse (Mm) 17-day embryos, mixed-stage C. elegans (Ce), Drosophila melanogaster (Dm) mixture of embryo-larvae-pupae, and in a sample of human heart (ht) tissue. Other human tissue samples were brain (br), liver (li), kidney (ki), and lung (lu). (E)mir-1 and mir-58 probes to total RNA from mixed populations of wild-type (+) and dcr-1(ok247) (-) animals. An increase in the proportion of unprocessed ~65-nt precursor is observed in the dcr-1 RNA.

Picture from Pelletier L et al. (2004) Curr Biol "The Caenorhabditis elegans centrosomal protein SPD-2 is required for both ...."

Figure 3. Centrosomal Distribution of SPD-2 during the First Cell Division and the Domain Structure of SPD-2 Homologs. (A) wt embryos after meiosis, at prometaphase, and at telophase were stained for DNA (not shown), SPD-2 (red), SAS-4 (green), and SPD-5 (blue). See Figure S2A for the stages analyzed. Merged images at the three different stages are also shown. Bar is 1 μm. Note that SPD-2 localizes to both the PCM and the centrioles.(B) Domain structure of C. elegans SPD-2 and multiple sequence alignment of the putative SPD-2 orthologs. SPD-2 contains a coiled-coil domain (aa 200-470, red box) and a SPD-2 domain (aa 484-714, green box) defined by similarity to its orthologs in other species. By comparing C. elegans and C. briggsae SPD-2, we identified six conserved CDK phosphorylation sites (consensus sequence: [ST]-P, indicated by black circles; aa positions 143, 171, 220, 233, 259 and 545 in C. elegans SPD-2). The putative phosphorylation site within the SPD-2 domain that is conserved in nematodes is not present in other eukaryotes. The conserved residues are boxed in green; the putative CDK phosphorylation sites are boxed in black with yellow lettering.(C) Multiple sequence alignment of the N-terminal region of the SPD-2 domain with the homologous region of vertebrate abnormal spindle proteins (Asp). Nearly all conserved residues present within the N-terminal region of the SPD-2 domain are also present in the N terminus of Asp proteins. Residues that are conserved in at least 87% of the sequences of the SPD-2 domain and Asp proteins are highlighted in yellow, those that are only specific for the SPD-2 domain are boxed in green, and those that are only specific for the Asp N-terminal domain are boxed in gray. Abbreviations: Hs, Homo sapiens; Dm, Drosophila melanogaster; Cb, Caenorhabditis briggsae; Ce, Caenorhabditis elegans; Gg, Gallus gallus; Xl, Xenopus laevis; and Dr, Danio rerio. Additional information about the alignments and accession numbers of the sequences used can be found in the Supplemental Data.