Picture from Muriel JM et al. (2005) Development "Fibulin-1C and Fibulin-1D splice variants have distinct functions and assemble ...."

Figure 7. Co-localization of fibulin-1D and hemicentin. (A-I) Hemicentin::GFP (A,D,G) and Fibulin-1D with an N-terminal YFP tag (B,E,H) co-localize (C,F,I) on ALM mechanosensory neurons (A-C), flexible tracks (D-F), and uterine attachments (G-I). Scale bar: 10 μm.

Picture from Huang X et al. (2003) Development "UNC-71, a disintegrin and metalloprotease (ADAM) protein, regulates motor axon ...."

unc-71 expression pattern. (F,G) UNC-71::GFP may be localized to the cell membrane. Punc-115UNC-71::GFP expression is seen in the nerve ring (arrow in F) and along the nerve cords(arrow in G). (H,I) Pe15*2UNC-71::GFP expression in hypodermis is seen near the nerve cords (arrow in H,I). Arrowhead in H indicates the vulva hypodermis. *Likely GFP aggregates. Scale bars: 20 um in F-I.

Picture from Dorfman MD et al. (2009) Genetics "Using RNA interference to identify specific modifiers of a ...."

Spinning-disk confocal projected Z-stack images of GFP fusion proteins driven by the GFP::C24D10.1 promoter. GFP::C24D10.1 expression (D-F, I). Bean stage (D), comma stage (E), 1.5-fold stage embryos (F), and head of adult around pharynx (I). Anterior is to the left. Bars, 10 um.

Picture from Schvarzstein M et al. (2006) Dev Cell "The C. elegans sex-determining GLI protein TRA-1A is regulated by sex-specific ...."

(B-I) (B, D, F, and G) Differential interference contrast micrographs and (C, E, H, and I) TRA-1 immunofluorescence of adult nematodes: (B and C) tra-1; eDp6[tra-1(+)] XX hermaphrodite and (D and E) a tra-1(null) mutant XX pseudomale sibling; (F and H) tra-1(+) XX hermaphrodite and (G and I) a tra-1(+) XO male sibling. Exposure times in (C) and (E) are identical, as are exposure times in (H) and (I).(J and K) DAPI staining of the animals in (F) and (G).

Picture from Klassen MP et al. (2010) Neuron "An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic ...."

Figure 5. ARL-8::YFP Is Strongly Enriched at the Presynaptic Terminal. (A-F) Colocalization of ARL-8::YFP (A and D) with mCherry::RAB-3 (B and E), double label (C and F) and high magnification (D-F), in DA9 (wyEx3198).(G-I) Enrichment of ARL-8::YFP (G) over cytosolic mCherry (H), quantified by a 1.4 μm moving average line scan (I) (wyEx3235). Scale bars, 10 μm.

Picture from Bembenek JN et al. (2007) Development "Cortical granule exocytosis in C. elegans is regulated by cell cycle components ...."

Figure 1. Identification of cortical granules. (A-C) Succynilated WGA-labeled cortical granules in C. elegans oocytes (A), and prometaphase I embryos (B), but not in embryos after meiosis I (C) (DNA in blue). (D-F) WGA-labeled cortical granules (D) are associated with SP12::GFP-labeled reticulate ER (E) at the cortex of a metaphase I embryo; merge in F. (G-I) UGTP-1::GFP-labeled 1 μm vesicles were clustered in prometaphase I (arrows, G) and redistributed across the cortex by metaphase I (arrows, H). Smaller cytoplasmic puncta remain after loss of the 1 μm vesicles (arrows, I). (J-L) WGA staining (J) co-localized with UGTP-1::GFP (K) in cortical granules in the cortex of a metaphase I embryo; merge in L. Scale bar: 10 μm.

Picture from Boag PR et al. (2005) Development "A conserved RNA-protein complex component involved in physiological germline ...."

Figure 4. CAR-1 colocalizes with CGH-1 in the embryo. Two-cell (A-C), four-cell (D-F), 16-cell (G-I), ~50-cell (J-L) and ~100-cell (M-O) embryos were immunostained for CAR-1 (A,D,G,J,M) and CGH-1 (B,E,H,K,N). Merged images that include DAPI staining are also shown (C,F,I,L,O). Anterior is to the left.

Picture from Sloan, Dillon E. et al. (2021) MicroPubl Biol

Figure 1. Endogenous HIS-72::linker::mTurquoise2: (A) Schematic of his-72 edit (B-F) Images of HIS-72::linker::mTurquoise2 localization: in a prometaphase I embryo (B, arrow indicates meiotic chromosomes), in an anaphase I embryo (C, arrow indicates meiotic chromosomes), at the distal gonad arm (D), in the proximal gonad arm (E), and in a 50-100 cell embryo (F). (G) HIS:-72::linker::mTurquoise2 in an adult worm.

Picture from Xiao H et al. (2009) Mol Biol Cell "Lysosome biogenesis mediated by vps-18 affects apoptotic cell degradation in ...."

Expression of VPS-18. (A) VPS-18::GFP expression in embryo (a and b), larva (c and d), adult coelomocyte (e and f), and germline (g and h) as well as the dissected-out germline (i and j). Arrows in (h and j) indicate germline sheath cells. The scale bars represent 20 um (a-d, i, and j), 5 um (e and f), and 10 um (g and h).

Picture from Peden EM et al. (2005) Curr Biol "The KLP-6 kinesin is required for male mating behaviors and polycystin ...."

Figure 5. Subcellular Localization of GFP-Tagged Full-Length, MOTOR, and C-TERMinal Domains of KLP-6. Confocal fluorescent images of cDNA KLP-6::GFP (A-C), C-TERM::GFP (D-F), and MOTOR::GFP (G-I) in male head (A, D, and G) and tail (C, F, and I). Cilia and axons are indicated by thin arrows and closed arrowheads, respectively. In (C), (F), and (I), the dashed box around the hook structure indicates HOB cilium.(A-C) KLP-6::GFP is distributed in a web-like pattern throughout neurons, including cilia. (B) Cilia of IL2 and CEM neurons shown at high magnification.(D-F) C-TERM::GFP is highly enriched in cilia of IL2s and CEMs and (F) RnBs and HOB. (D and F) Nuclear localization of C-TERM::GFP is also observed.(G-I) MOTOR::GFP is preferentially targeted to cell bodies and axons and is largely missing from cilia. (H) High magnification of IL2 and CEM cell bodies and axons from (G). Anterior-posterior (a-p) axis is indicated. Open arrows indicate filamentous, cage-like structures around IL2 cell bodies labeled by MOTOR::GFP. The scale bars represent 5 μm.