(B-D) Representative images of live embryos (B,C) and fixed late L1 larva (D) expressing
let-381::gfp. (E-N) The left side of three L1 larvae double-labeled with LET-381::GFP (E,I), anti-FOZI-1 antibody (F,J) and DAPI (H) at the 14-M (E-H) and 16-M stage (I-K). (G,K) Merged images of E and F and of I and J, respectively. (L-N) An L1 larva double-labeled with anti-LET-381 (L) and anti-FOZI-1 antibodies (M) at the 16-M stage. (N) A merged image of L and M. (O) Summary of LET-381 expression in the M lineage, with LET-381-positive cells in green circles and CEH-34-positive cells outlined in black.