Figure 1. Characterization of
apl-1 expression and loss of function. (A(a-d)) DIC images corresponding with APL-1::GFP fluorescence (A(e-h))respectively. vpi - pharyngeal intestinal valve; VC - ventral cord; exc- excretory cell; utse - uterine seam junction (Scale bar, 10 um) (B) N2 L4 worm on control RNAi bacteria (L4440) (a), L4 worm with partial gene knock-down using
apl-1 RNAi showing loose cuticle in the head (b) and tail (c) region.(Scale bars, 20 mm) (C) N2 worm at L1 (a) compared to an
apl-1(
tm385) homozygous L1 arrest demonstrating degradation in the head (b) and tail (c).(Scale bar, 10 mm) (D) Rescue of the molting phenotype of the
apl-1 homozygous null worms by
apl-1::gfp at L1 (a) and L4 stages (b). (Scale bars10 um and 20 um respectively).