Picture from Robinson-Thiewes, Sarah et al. (2021) MicroPubl Biol

Figure 1. The mpk-1b long first intron enhances MPK-1B translation: (A) The mpk-1 locus generates two RNAs, mpk-1b and mpk-1a; for simplicity, only mpk-1b is shown with its exclusive first exon and 8 kb long first intron. Deletions within the long first intron were generated in the dual tagged (DT) mpk-1 locus, which carries 1XV5 to mark MPK-1B specifically, and 2XOLLAS to mark both MPK-1A and MPK-1B (Robinson-Thiewes et al. 2020a).

Picture from Gravato-Nobre MJ et al. (2016) PLoS Pathog "The Invertebrate Lysozyme Effector ILYS-3 Is Systemically Activated in ...."

(J-K) Representative images of the 4.5 Kb ilys-3 long promoter GFP construct in adult hermaphrodites. (J) Promoter activity in pharynx and intestine and (K) in the coelomocytes. ph:pharynx; int: intestine; cc: coelomocytes. Scale bars as indicated.

Picture from Gaiser AM et al. (2011) PLoS One "Downregulation of the Hsp90 system causes defects in muscle cells of ...."

Figure 2. daf-21 is ubiquitously expressed in Caenorhabditis elegans. A-C. Nematodes of the BC10293 strain, which contains a transcriptional fusion of GFP to the daf-21 promoter are shown. Fluorescence can be detected in the excretory canal cell (long arrow), in the pharyngeal muscle cells (sharp triangle) and the cells of the first intestinal ring (asterisk). Further individual muscle cells (blunt triangle) and the vulval muscles (short arrow) show fluorescence (A). Detailed views of the same strain highlight the excretory canal cell (long arrow), an individual muscle cell (blunt triangle) (B), the pharyngeal muscle cells (sharp triangle) and the cells of the first intestinal ring (asterisk) (C). D-E. Newly generated transgenic nematodes, carrying a daf-21 promoter sequence fused to YFP. Fluorescence is detected in the excretory canal cell (long arrow) and body wall muscle cells (wide triangles) (D). Fluorescence is also pronounced in some head neurons (E). The scale bar represents 40 mm in all subpanels.

Picture from McMahon L et al. (2001) J Cell Sci "Assembly of C. elegans apical junctions involves positioning and compaction by ...."

Confocal images showing an external (D) and internal (E) focal plane of a 1.5-fold dlg-1::gfp transgenic embryo after immunostaining with anti-GFP antibody. Weak background fluorescence could often be seen around the pharyngeal lumen and rectum (arrowheads in E; these expressing cells have not been identified). (F-H) GFP fluorescence of a young dlg-1::gfp transgenic adult showing expression in epithelial cells of the pharynx (arrow in F), intestine (arrowhead in F and large arrow in H), vulva (large arrow in G), uterus (arrowheads in G), spermatheca (long arrows in G), rectum (long arrow in H) and epidermis (arrowhead in H). Scale bars, 50 um (F-H); 10 um (D,E).

Picture from Trzebiatowska A et al. (2008) Mol Biol Cell "Caenorhabditis elegans teneurin, ten-1, is required for gonadal and pharyngeal ...."

Figure 8. The long TEN-1 isoform is expressed in the developing and adult pharynx. The GFP::TEN-1 transgene (kdEx121) is expressed in the developing pharynx of the early embryo (A and B) and outlines the adult pharynx (C and D). Expression of the kdEx121 is also found in some head neurons (D). Scale bar, 20 um.

Picture from Du H-P et al. (1996) Neuron "Extracellular proteins needed for C. elegans mechanosensation."

Figure 8. Beta-Galactosidase Expression from mec-9-lacZ Fusions. All animals are L4 larvae.(a) Long transcript expression in the touch receptor neurons from TU#165.(b) Short transcript expression from TU#160 in some ventral cord motor neurons and cells in the head.(c) Expression from both transcripts using TU#171. Both sets of cells stain.

Picture from Lee, Bo Yun et al. (2022) MicroPubl Biol

Figure 1. The structure of TALT1 arrays in CB4856 and DL226(A) Schematic representation of chromosome V right arm regions in N2, CB4856, and DL226. Locations of TALT sequences are V:21149554-21162688 in the CB4856 genome and V:21278369-21293965 in the DL226 genome. Six raw reads covered the 6-copy TALT array of DL226. DL226-specific TALT insertion was indicated by a gray horizontal line. The six raw-read sequences are available in Extended Data 2. (B) The comprehensive structure of duplicated regions. The new telomeric repeat and TALT (the long purple line) are right next to the original copy (the long green line). Besides, a duplicated part of telomeric repeat (the short purple line) is also in the repeat.

Picture from Jee C et al. (2012) J Mol Biol "CNP-1 (ARRD-17), a novel substrate of calcineurin, is critical for modulation of ...."

Fig. 3. Expression patterns of cnp-1. cnp-1 is expressed mostly in neurons from embryo (a) to adults (b-m). Transgenic worms expressing cnp-1 promoter::gfp [pCH7] [all but (d)] constructed in a wild-type background and a CNP-1::GFP cDNA construct [pCH13] rescuing cnp-1(jh105) mutant worms (d) are shown. (a) A threefold embryo showing GFP in head and tail regions. (b) Lateral view of an adult expressing GFP in head neurons. Amphid socket cells (arrow heads), CEP neurons (long arrow) and nerve ring (short arrow). (c) Ventral view of vulva region. HSN neuron (arrow heads) and vulva (short arrow) and ventral nerve cords (long arrow). (d) Rescue of cnp-1(jh105) mutant by microinjection of cloned CNP-1::GFP construct [pCH13]. Nerve ring (arrow head), ventral nerve cords (long arrow) and tail ganglia (short arrow). (e-g) Merged confocal image stacks of GFP expression (green) and DiI dye (red), showing cnp-1 expression in ASK, ADL, ASH and ASJ as indicated. (h-m) Single confocal plane showing that the individual neuron is presented. Scale bar in (a) represents 50 um, and other scale bars represent 20 um.

Picture from Weinshenker DZ et al. (1999) J Neurosci "Block of an ether-a-go-go-like K(+) channel by imipramine rescues egl-2 ...."

Figure 5. egl-2 expression. Anterior is left, dorsal is up. Merged Nomarski and GFP fluorescence images.a, Short EGL-2::GFP expression in the intestinal muscles (posterior end of an adult animal). GFP-expressing cells posterior to the intestinal muscle are the tail neurons ALN and PLN in a slightly lower focal plane. b, Short EGL-2::GFP expression in neurons in the lateral ganglion (anterior end of the animal). Posterior to the IL2 neurons, BAGL and AWCL are also visible, but faint. Other GFP-expressing cells in this region are either faint or out of focus. c, Long EGL-2::GFP expression in the ALM mechanosensory neurons.d, Long EGL-2::GFP expression in the vulval muscles used for egg laying. e, Deconvoluted image of full-length EGL-2::GFP fusion expression in sensory neuron endings.

Picture from Grisoni K et al. (2003) J Mol Biol "The stn-1 syntrophin gene of C.elegans is functionally related to dystrophin and ...."

Figure 3. stn-1 is expressed in muscles and neurons. Expression of stn-1 was assayed by reporter gene analysis. Fluorescence microscopy images of wild-type animals carrying a stn-1:gfp transgene. A, Expression in a twofold embryo seen with a fluorescence microscope (630). GFP fluorescence is detected in the head neurons and muscles. B-E, Expression in a adult hermaphrodite seen with a confocal microscope. B, Projections of series of ventral views (400). Staining is visible in the nerve cord (long arrow) and muscles (short arrow). C, Lateral view of the vulva showing intense staining in the vulva muscles (630). D, Staining of body wall muscle cells (630). E, Projections of series of ventral views (630). Staining is visible in the nerve cord (long arrow), body-wall muscles (short arrow), and vulva muscles (vertical arrow).