Figure 5. lf mutation of
unc-103,
dgk-1, or
eat-16 did not augment neurotransmitter release either in the presence or in the absence of
unc-43(gf). The mutant alleles used were
unc-103(
n1213),
dgk-1(
sy428),
eat-16(
sa609), and
unc-43(
n498). A, Representative traces of mPSCs (top) and ePSCs (bottom). B, Comparison of mPSC frequency, mPSC amplitude, and ePSC amplitude. All values are shown as the mean ± SE. One-way ANOVA with Bonferroni's post hoc tests was used to compare between wild type and each of the three single mutants (open columns), and between
unc-43(gf) and each of the three double mutants (filled columns). The asterisk indicates statistically significant difference compared with the wild type (WT) (p < 0.05). There was no significant difference among the groups containing
unc-43(gf). The number of samples analyzed is indicated inside each column. C,
unc-103 expression along the ventral cord was restricted to GABAergic motor neurons. The
unc-103 expression pattern was evaluated by expressing Punc-103::GFP. GABAergic neurons were labeled by expressing Punc-47::RFP. Yellow-colored neurons in the merged pictures are GABAergic neurons that express
unc-103. The left panels show images of a young adult (scale bar, 100 μm), whereas the right panels show images of a larva (scale bar, 50 μm).
unc-103 expression was observed in many head neurons (arrow), GABAergic motor neurons (asterisks), and the vulval muscle (arrowhead). For clarity, GABAergic motor neurons of the young adult are not labeled. DIC, Differential interference contrast.