Figure 6. Native and heterologous expression of the
gar-3::YFP translational fusion protein. A, B, DIC (A) and fluorescence (B) images of the left lateral tail region of a mid L4 male expressing both Pgar-3A::
gar-3::YFP (pYL8) and Pgar-3B::
gar-3::YFP (pYL9). Nuclei of the neurons are labeled with arrows. In B, positions of these neurons are indicated with circles, and localizations of GAR-3::YFP on the cell membranes are denoted by arrows. C, D, DIC (C) and fluorescence (D) images of left lateral tail region of an adult male expressing Punc-103 E::
gar-3::YFP (pYL10). E, F, Merged DIC and fluorescence (E) and fluorescence (F) images of left lateral tail region of a mid L4 male expressing the Punc-103 F ::
gar-3::YFP (pYL11). In E, nuclei of neurons are labeled with arrows. GAR-3::YFP localization on cell membranes can be seen as white puncta. In F, neuronal positions are indicated with circles, and localizations of GAR-3::YFP on the cell membranes are denoted by arrows. Scale bar, 10 μm.