Picture from Ow MC et al. (2008) Genes Dev "The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic ...."

Expression pattern of FLH-3. The expression of a GFP transcriptional reporter for flh-3 (Pflh-3::gfp) displayed the most intense expression during late stages of embryogenesis and little GFP in mid-stage larvae. Expression from Pflh-3::gfp was also detected in L1 larvae.

Picture from Loer CM et al. (2015) Genetics "Cuticle integrity and biogenic amine synthesis in Caenorhabditis elegans ...."

(D) qdpr-1 reporters. Top images: single optical sections from larva showing broad epidermal expression, but little expression in neurons; strain CZ19213. Insets: enlargements of midbody regions. Bottom image: Head showing expression in identified 5HT and DA neurons: NSMs, ADFs, CEPs, and other cells, with reduced epidermal expression; strain CZ19215 (lateral view, MIP).

Picture from Ezechukwu, Chiemekam Samuel et al. (2022) MicroPubl Biol

Figure 1. Cytological and phenotypic characterization of cosa-1:mCherry(wsh7):(A) Fluorescence images showing the localization of the COSA-1::mCherry foci in the different zones of the germline. White rings indicate examples of 6 COSA-1::mCherry foci per nuclei in the late pachytene (LP) of adult worms, blue arrows define germline distal to proximal direction (MZ - mitotic zone, TZ - transition zone, EP - early pachytene, MP - mid pachytene, LP - late pachytene, DP -diplotene). (B) Colocalization of GFP::COSA-1 and COSA-1::mCherry. (C - E) Analysis of brood size (C), progeny viability (D) and male frequency (E) in the cosa-1::mCherry(wsh7) transgenic strain compared with the wild type (ns - not significant; Independent samples t-test, n= 26).

Picture from Li Q et al. (2018) PLoS Genet "The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex ...."

Fig 2. GFP::BRC-1 and BRD-1::GFP localize to the SC in meiotic prophase. A) Nuclei from indicated meiotic stages stained withRAD-51 antibodies (red), DAPI (blue) and imaged for GFP fluorescence (green). White arrows demark foci positive for both GFP fluorescence and RAD-51 signal; green arrows demark foci containing GFP but not RAD-51; red arrows demark foci containing onlyRAD-51. Scale bar = 5 um. B) Co-localization between GFP::BRC-1 (green) and SC central component SYP-1 (red) by antibody staining;germ lines at indicated stages were counterstained with DAPI. Blue arrows at late pachytene show chromosomal regions where GFP::BRC-1 concentrates before SYP-1. Scale bar = 2 um. C) TagRFP-T::BRC-1 (red) and GFP::COSA-1 (green) at late pachytene showingTagRFP-T::BRC-1 on one side of the GFP::COSA-1 focus, which marks the persumptive crossover. Scale bar = 2 um. Images areprojections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene;DP = diplotene; DK = diakinesis.

Picture from Colbert HA et al. (1997) J Neurosci "OSM-9, a novel protein with structural similarity to channels, is required for ...."

C, A lateral view of the head of a transgenic animal expressing the osm-9:: GFP3 fusion gene, which encodes a GFP-tagged OSM-9 protein. GFP is localized to the OLQ and AWA cilia at the tip of the nose (arrows), but little GFP is present in the cell bodies, axons, or dendrites. ADL expression is weaker or absent in these lines. The fusion gene deletes the last 105 amino acids of OSM-9; when GFP is fused to the last amino acid of OSM-9 (osm-9:: GFP4), a similar but fainter expression pattern is observed.

Picture from Labella S et al. (2011) Dev Cell "Polo kinases establish links between meiotic chromosomes and cytoskeletal ...."

Figure 2. PLK-1 Partially Colocalizes with PLK-2 Transition zone nuclei from wild-type and plk-2 mutant germlines stained with PLK-1 (red) and (A, B, and D) PLK-2 (green) or (C) HTP-3 (green). (A and B) PLK-1 colocalizes with PLK-2 to the nuclear periphery in some NE associated patches but is undetectable in others (arrows). (C and D) In the absence of PLK-2, bright PLK-1 signals can be detected at four to five NE-associated patches; however, little PLK-1 can be detected on the synapsing chromosomes, in comparison to PLK-2 (compare to Figure S4). Scale bars, 5 um. See also Figure S2.

Picture from Kraemer B et al. (1999) Curr Biol "NANOS-3 and FBF proteins physically interact to control the sperm-oocyte switch ...."

(a) Expression of nos-3 mRNA. Polyadenylated C. elegans mRNA was separated through a 1% agarose glyoxal gel, transferred to a filter and probed with a nos-3 cDNA. Lane 1, wild-type adults, which possess approximately 2,000 germ cells; lane 2, glp-1(q224) mutant adults, which possess no more than eight germ cells; lane 3, mixture of developmental stages (from first larval stage through adults with embryos); lanes 4-9, embryos (E), the four larval stages (L1-L4) and adults (A), respectively. In lane 2, note that very little nos-3 mRNA is detectable; the small amount remaining may represent somatic expression. To verify equal loading of lanes, the blot was probed for expression of elongation factor-1 alpha (eft-3).

Picture from Speliotes EK et al. (2000) Mol Cell "The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase ...."

Figure 1. BIR-1 Expression Correlates with Cell Divisions. (A) Total protein extracts from wild-type No RNAi or bir-1(RNAi) embryos were stained with affinity-purified anti-BIR-1 antibody.(B) Protein extracts from synchronized wild-type worm populations were immunoblotted and probed with affinity-purified anti-BIR-1 antibody. E, embryos; L1, L2, L3, and L4, larval stages 1, 2, 3, and 4; and A, adults.(C) Fixed embryos were stained with anti-BIR-1 or anti-a-tubulin antibodies to visualize BIR-1 (green) and a-tubulin (red), respectively, and counterstained with DAPI to visualize DNA (blue). The top row shows an early embryo in which cells were dividing. Arrows point to BIR-1-positive structures. The bottom row shows an older embryo (500 cells) in which cells were starting to differentiate and little BIR-1 staining was detectable. Scale bar, 20 um.

Picture from Paupard MC et al. (2001) J Histochem Cytochem "Immuno-EM localization of GFP-tagged yolk proteins in C. elegans using ...."

Figure 4. The yolk receptor protein (RME-2) localizes to different cellular compartments in full grown oocytes and in embryos of wild-type adult. (A) Anti-RME-2 antibodies (EXT) label a pool of receptors near the plasma membrane of two mature oocytes. Double arrowheads indicate the parallel borders of two neighboring oocytes running left to right. Most gold label falls very close to this border region, but relatively little is associated with yolk granules except very near the cell membrane. (B) Anti-RME-2 antibodies (EXT) label receptors away from the plasma membrane in a fertilized oocyte. The gold label now clusters over electron-dense clumps deeper within the cytoplasm and is rarely adjacent to yolk granules. Arrowheads indicate the plasma membrane of the embryo, running left to right just above the eggshell at bottom of panel. Anti-RME-2 was used at dilutions of 1:10 to 1:50 on tissues fixed in 4% paraformaldehyde by the microwave fixation protocol. Bars 500 nm.

Picture from Stamper EL et al. (2013) PLoS Genet "Identification of DSB-1, a protein required for initiation of meiotic ...."

Figure 4. DSB-1 localizes to chromosomes during early meiotic prophase. (A) Composite projection image of a gonad from a WT animal showing DAPI and immunofluorescence staining for DSB-1 and RAD-51. Scale bar, 30 mm. (B) Partial projection image of pachytene meiotic nuclei from WT showing DAPI and immunofluorescence staining of DSB-1. Scale bar, 5 mm. (C) Immunofluorescence staining of DSB-1 and HIM-8 in early pachytene nuclei in WT. HIM-8 was used to mark the X chromosome (arrows). DSB-1 staining was reduced on the X chromosome. (D) Partial projection image of early pachytene nuclei in WT showing immunofluorescence staining of RAD-51 and DSB-1. Little colocalization was observed between DSB-1 and RAD-51. (E) Partial projection image of early pachytene nuclei from WT showing immunofluorescence staining of DSB-1, HTP-3, and DAPI. Only a subset of DSB-1 colocalized with chromosome axes, marked by HTP-3. Scale bar, 5 um.