Picture from Pauli F et al. (2006) Development "Chromosomal clustering and GATA transcriptional regulation of ...."

Figure 3. Intestinal expression of six genes identified by mRNA tagging. The anatomical expression of six genes chosen from the list of 1938 intestinal genes was visualized by transformation with promoter::GFP transcriptional constructs. B0218.8 (clec-52), C25E10.8, ZK970.2 (clpp-1), elo-6 and gst-42 show exclusive intestinal expression and D2030.5 (mce-1) shows expression in the intestine as well as other tissues.

Picture from Wenick AS et al. (2004) Dev Cell "Genomic cis-regulatory architecture and trans-acting regulators of a single ...."

Figure S7. Expression Pattern of All New Reporter Genes Tested. Numbers indicate ranking in the high-score list. Gene names in red are aminergic/peptidergic 7TMRs; gene names in blue are ligand-gated ion channels. gfp fusions to genes that are expressed in AIY are boxed in red. Upper panels each show gfp expression, lower panel the overlap with AIY-expressed rfp transgene otIs133.

Picture from Kuhara A et al. (2008) Science "Temperature sensing by an olfactory neuron in a circuit controlling behavior of ...."

Figure S2. Expression pattern of eat-16 and promoters for rescue experiments. (A) Cells expressing eat-16::gfp in wild-type. Yellow bar indicates 10 micrometers. (B) List of cells expressing GFP fluorescence driven by fusion genes encoding the indicated promoters and gfp gene (promoter::gfp). The eat-4, odr-3, gpa-13, odr-1 promoters all drive gene expression in AWC, although these promoters drive gene expression in different sets of neurons.

Picture from Grossman EN et al. (2013) Genetics "Mechanisms of ephrin receptor protein kinase-independent signaling in amphid ...."

Asymmetry in Pefn-2- mCherry (juEx2737) expression as determined by 4D lineage analysis at 335 min postfertilization; anterior is to the left and left is up. Blue nuclei are bilateral pairs that show symmetrical expression of efn-2, green nuclei are members of bilateral pairs for which only the right-hand side expresses efn-2, red nuclei are members of pairs that express only on the left-hand side. Gray nuclei are nonexpressing (grayscale depth code). See Table S2 for list of expressing nuclei.

Picture from Cohen, Sarah M et al. MicroPubl Biol

Figure 1. Transcriptional response to ascarosides of C. elegans in early L1 stage: (a) The top ten up-regulated and (b) down-regulated genes in N2 (wild-type) L1 larvae. (c) The top ten up-regulated and (d) down-regulated genes in daf-22 mutant L1 larvae. (e) A heatmap of the most significant genes at p = 0.001 with annotated biological functions, derived from WormBase Gene Ontology annotations, in the N2 and daf-22 samples. A full list of the significant genes at p = 0.001 can be found in the Extended Data.

Picture from Kuramochi, Masahiro et al. (2023) MicroPubl Biol "The effect of ice-binding proteins on the cryopreservation of Caenorhabditis ...."

Figure 1. Recovery rates of control animals and IBP-expressing worms:(A) Experimental procedures to observe the freeze and cold tolerance in living C. elegans (Kuramochi et al. 2019; Kuramochi et al. 2022b).(B) Experimental procedure to observe the recovery rate of C. elegans after cryopreservation.(C) Strain list.(D) Recovery rate of control animals and each IBP-expressing worm after cryopreservation at −80°C. In each assay, n > 100 (group = 5). Boxes show the first, second and third quartiles. The Wilcoxon rank sum test with Bonferroni correction was performed. *p < 0.05.(E) Detailed information on the recovery rate in (D).

Picture from Stetak A et al. (2009) PLoS One "Neuron-specific regulation of associative learning and memory by MAGI-1 in C. ...."

Figure 2. Expression pattern and sub-cellular localization of MAGI-1::GFP. a, expression of the rescuing magi-1::gfp construct in the head of an adult worm visualized by fluorescence confocal microscopy. b and c, list of the neurons expressing MAGI-1. MAGI-1 and GLR-1 double positive cells are indicated in bold. Neurons that project their processes along the ventral nerve chord are listed in c. d-f, co-localization of MAGI-1::YFP with the presynaptic marker, synaptobrevin (SNB-1) driven by endogenous promoters. Asterisks label the synaptic area, arrows point to the punctae flanking the synaptic cleft. Scale bars represent 10 µm on panel a, and 1 um on panel f.

Picture from Stitzel ML et al. (2007) Curr Biol "Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the ...."

Figure 7. EGG-3 Is a Likely Target of the Anaphase-Promoting Complex/Cyclosome.(A) Amino acid sequence of EGG-3. PTP domain is underlined, D boxes are marked in red, and the phosphatase active site is green.(B) List of mutants tested for effect on cortical localization and degradation of GFP:EGG-3.(C) Images of live embryos expressing GFP fusions as indicated.(D) Western showing similar expression levels of the wild-type and mutant GFP:EGG-3 fusions. The increased stability of some of the fusions, as shown in (C), does not result in detectably higher levels in the western, possibly because the increase in 2-cell and later stages is modest compared to total EGG-3 present in oocytes and early zygotes.

Picture from Dour, Scott et al. MicroPubl Biol

Figure 1. Expression levels of transgenic insertions with altered regulatory elements: Quantification of GFP expression in ALM and PLM soma of L4 animals with homozygous single copy insertions of constructs schematized on the left. The mec-4 promoter is shown in olive green, GFP in bright green, and 3' UTRs in other colors. Modifications to the promoter and GFP are highlighted in red. Individual measurements (filled grey circles) and the mean (black bar) are shown. The units are defined identically for ALM and PLM measurements. See strain list for the exact genotype of animals analyzed. All of the transgenes also express in AVM and PVM. None of the transgenes express in any other cell types to detectable levels. n=13-19 for ALM and 26-39 for PLM.

Picture from Nonet, Michael (2021) MicroPubl Biol

Figure 1. GAL4SK and TetR bipartite driver activity improves with a flexible linker: Quantification of GFP expression of various GAL4SK and TetR bipartite drivers with distinct linkers separating the DNA and activation domains. The mec-4 promoter was used to express the drivers. An 11X UAS &#x2206;pes-10p GFP-C1 reporter was used to assay GAL4SK activity and a 7X tetO &#x2206;pes-10 GFP-C1 reporter was used to assay TetR activity. See strain list for the exact genotype of animals analyzed. Individual measurements (filled grey circles) and the mean (black bar) are shown. The units are defined identically for ALM and PLM measurements. All of the transgenes also express in AVM and PVM. None of the transgenes express in any other cell types to detectable levels. Strains used: NM5225, NM5467, NM5468, NM5301, NM5470, NM5233 and NM5362. n=15-17 for ALM and 30-34 for PLM. T-test: p*<0.01, ** p<0.0001.