Picture from Kipreos ET et al. (1996) Cell "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene ...."

Figure 5. Expression of Cullin mRNAs. (A) Northern blot of total RNA from wild-type embryos (E), larvae (L1, L2, L3, L4), and pregravid adults (A). The same membrane was probed sequentially with cul-1, cul-2, cul-3, cul-4, and 18S rRNA cDNAs.(B) Northern blot of poly(A)-selected RNA from mature wild-type, fem-1(hc17), and glp-4(bn2) adults cultured at 25 C. The membrane was probed sequentially with cul-1 and, for comparison, with cey-2 and act-1 cDNAs. In wild type, cey-2 mRNA is found only in germline cells and developing embryos (W. Kelly, personal communication).

Picture from Malone CJ et al. (1999) Development "UNC-84 localizes to the nuclear envelope and is required for nuclear migration ...."

Figure 6. Functional UNC-84A::GFP is ubiquitously expressed and localized to the nuclear periphery. (A) A fluorescence and (B) a Nomarski image, of neuronal, hypodermal, uterine and intestinal nuclei expressing UNC-84A::GFP. All somatic nuclei shown express the reporter construct. Bar, 10 µm. (C) A fluorescence and (D) a Nomarski image, of the lateral surface of an UNC-84A::GFP expressing larva showing ubiquitous expression in hypodermal cells. Bar, 10 µm. (E) A fluorescence and (F) a Nomarski image, of a migrating distal tip cell (white arrow). UNC-84A::GFP does not appear to be expressed in the germline, as is commonly observed for C. elegans transgenes (Kelly et al., 1997). Bar, 5 µm.