over-expressed (OE) TSP-12::GFP (I, K, M, O) in the early embryo (A), L4 developingvulva (C), late L3 hypodermis (E), young adult gonad (G), L3 larva head (I), L4 intestine (K: basolateral focal plane of the intestine and the arrow points to the lateral membrane, M: middle focal plane of the intestine andthe arrow points to the apical lumen of the intestine), and L3 neurons (O). (Q and R) L1 transgenic animalscarrying both over-expressed TSP-12::GFP and the M lineage specific reporter
hlh-8p::NLS::mCherry. TSP12::GFP is present in M lineage cells from 1-M (Q) to 8-M (R) stages. Some M lineage cells are out of the focalplanes shown. Scale bars represent 10um in A-R. (S) Rescue of the small body size of
tsp-12(
ok239);
tsp-14(
jj95) worms produced by
tsp-12(
ok239)/+;
tsp-14(
jj95) mothers using tissue-specific
tsp-12 cDNAexpression. The mean body length of WT worms is normalized to 1.0. Error bars represent 95% confidenceintervals for the normalized body length.