Picture from Linder B et al. (1996) J Biol Chem "Molecular characterization of a novel, developmentally regulated small ...."

Figure 5. Effects of stress and stage of development on expression of SEC-1 mRNA. Northern blots that contain size-fractionated RNAs from C. elegans were prepared as described under Experimental Procedures. Blots were probed with 32P-labeled SEC-1 cDNA corresponding to nucleotides 58-319 in Fig. 1. Hybridization of the probe with (0.65 kb) SEC-1 mRNA was visualized by autoradiography. A, fractionated RNAs (4 μg) from control C. elegans (lane 2) and C. elegans incubated with 0.2 mM CdCl2 for 24 h (lane 1) were analyzed. B, lanes of a Northern gel received 4 μg of total RNA from either embryos (E) or larvae (L1-L4) at various stages of postembryonic development. C (upper part), Northern blot analysis was performed on samples of total RNA (20 μg) isolated from control C. elegans maintained at 20 C (lane 3) or heat-stressed nematodes that were incubated for 3 h (lane 1) or 1 h (lane 2) at 31 C. The lower part shows a portion of the same blot that was hybridized with a 32P-labeled cDNA probe for myosin light chain mRNA, as described previously. Levels of the two myosin light chain transcripts do not vary with stress or development (not shown). Thus, the resulting signals document the integrity of the RNA samples and show that equal amounts of RNA were loaded in each lane.

Picture from Linder B et al. (1996) J Biol Chem "Molecular characterization of a novel, developmentally regulated small ...."

Figure 8. Developmental regulation of SEC-1 expression in C. elegans. A, samples of proteins (40 μg) isolated from C. elegans embryos (E), L1-L4 larvae and egg-laying adults (EL) were assayed for SEC-1 expression by Western immunoblot analysis as described in Fig. 7 and Experimental Procedures. The lane marked Std contained 50 ng of the recombinant SEC-1 polypeptide. B, samples of proteins (40 μg) from embryos (E) and L2-L4 larvae were assayed for the expression of the 40-kDa catalytic subunit of protein kinase A by Western immunoblot analysis as described previously (46). The lane marked Std received 60 ng of purified catalytic subunit.

Picture from Linder B et al. (1996) J Biol Chem "Molecular characterization of a novel, developmentally regulated small ...."

Figure 9. Detection of SEC-1 mRNA and protein in situ. For A and B, C. elegans were fixed, permeabilized, and then hybridized with digoxigenin-labeled, SEC-1 antisense DNA as described previously (55). RNA-DNA complexes were visualized by incubating the specimens serially with antidigoxigenin IgGs coupled to alkaline phosphatase and a chromogenic substrate. Alkaline phosphatase catalyzes the synthesis of an insoluble reaction product (shown as a black precipitate) in cells expressing SEC-1 mRNA. A, immature embryos (arrows) stain intensely for SEC-1 mRNA. Embryos that have undergone morphological transitions that result in the development of the classical C. elegans body shape (arrowheads) have low or undetectable levels of SEC-1 mRNA. B, L4 larvae and adult (not shown) C. elegans selectively express SEC-1 mRNA in developing oocytes in the gonad. Specimens in A and B were photographed using Nomarski interference optics with a Zeiss Axioscop microscope as described previously (40). C, C. elegans embryos were fixed, permeabilized, and incubated with antibodies directed against SEC-1 as described previously (45). Subsequently, samples were incubated with fluorescein isothiocyanate-coupled goat IgGs directed against rabbit immunoglobulins (45). Fluorescence signals corresponding to SEC-1-IgG complexes were obtained with a Bio-Rad MRC 600 laser scanning confocal microscope as described previously (45). Immature embryos (arrows) contain substantial levels of SEC-1 protein, whereas nematodes undergoing morphogenesis stain weakly (arrowheads) or remain unstained (10 additional mature embryos, which are observed in this field by phase microscopy, are not visualized by immunofluorescence).

Picture from Linder B et al. (1996) J Biol Chem "Molecular characterization of a novel, developmentally regulated small ...."

B, lanes of a Northern gel received 4 μg of total RNA from either embryos (E) or larvae (L1-L4) at various stages of postembryonic development.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(B, B') Pgrd-5::GFP is expressed in a pharyngeal neuronal cell.

Picture from Gunderson, Emma et al. MicroPubl Biol

Results of motility screens of C. elegans adults (A) in the wMicroTracker, B. pahangi females (B) & B. pahangi males (C) in both the WormAssay and the wMicroTracker, and B. pahangi microfilariae (D) , B. pahangi L3 (E) and S. mansoni schistosomules (F) in the wMicroTracker. Data are presented as mean +/- 95% CI.

Picture from Shen X et al. (2005) PLoS Genet "Genetic interactions due to constitutive and inducible gene regulation ...."

(B) atf-6-promoted GFP.

Picture from Dejima K et al. (2010) J Biol Chem "Two Golgi-resident 3'-Phosphoadenosine 5'-phosphosulfate transporters play ...."

Subcellular localization of PST-2. Confocal images of (B) anti-GFP antibody staining and (B') anti-DsRed antibody staining in the intestine of a transgenic worm carrying pst- 2(fl)::egfp and Paman-2::aman-2::mCherry transgene constructs. The intestine is enclosed in the dashed lines. (B'') Merged B and B' images. The area within the box in B'' was enlarged for B'''. Scale bar = 20 mm. (C) Quantitative comparison of the Golgi localization of PST-1 and PST-2. The percentages of co-localization with PST proteins and Golgi marker are shown. 2

Picture from Zhao G et al. (2007) Genome Res "Identification of muscle-specific regulatory modules in Caenorhabditis ...."

(B) C10G11.7::gfp expression in neurons.

Picture from Loria PM et al. (2003) Curr Biol "Two neuronal, nuclear-localized RNA binding proteins involved in synaptic ...."

(B and C) Expression of promoter fusions in embryos at the (B) 300-cell stage and in (C) L1 larvae.