Picture from Chamberlin HM et al. (2000) Development "The bromodomain protein LIN-49 and trithorax-related protein LIN-59 affect ...."

Figure 3. lin-59::gfp expression. (A) Nomarski photomicrograph of an L1 transgene-containing animal. (B) Fluorescence image, showing widespread expression of lin-59::gfp. Scale bar, 20 um.

Picture from Zhang X et al. (2011) Genetics "Spatial regulation of lag-2 transcription during vulval precursor cell fate ...."

Figure 6 LIN-1::GFP is uniformly expressed in all VPCs. (A)The lin-1 genomic region, with the position of gfp insertion.(B) GS5958, carrying the fosmid-based lin-1::gfp reporter,shows LIN-1::GFP expression in all VPCs before (L3Pn.p) and after induction (Pn.px).

Picture from Moskowitz IPG et al. (1996) Development "lin-12 and glp-1 are required zygotically for early embryonic cellular ...."

lin-12 expression in the early embryo, immunofluorescence micrographs. Bar, approximately 10 um. lin-12::lacZ expression at the 55- cell stage. Shown is a lin-12::lacZ; smg-1 transgenic embryo stained with anti-b-galactosidase antibodies in a dorsal view with anterior to the left. Robust b-galactosidase expression is observed in 24 cells.

Picture from Shih, Mushaine et al. MicroPubl Biol

Figure 1. Broad lin-41 expression in the C. elegans nervous system at the third larval stage: (A) NeuroPAL markers were used to identify lin-41-expressing neurons based on a Plin-41::gfp reporter expression in the head, anterior midbody, posterior midbody, and tail regions. (B) lin-41 expression in DA/B motor neurons was verified using a Punc-129::gfp reporter. Arrowheads point to DA/B motor neurons. A CRISPR-engineered reporter strain with the endogenous lin-41 gene tagged (lin-41(xr76) [mNG::LIN-41]) was used to identify perinuclear expression of LIN-41 proteins in a pair of AWC olfactory neurons (AWCleft and AWCright ) (C), and FLP and PVD nociceptive neurons (D). Scale bars represent 20 μm.

Picture from Bettinger JC et al. (1996) Development "Stage-specific accumulation of the terminal differentiation factor LIN-29 ...."

(B) Wild-type adult stained with anti-LIN-29 and MH27 antibodies and visualized by indirect immunofluorescence. LIN-29 has accumulated in the seam cell nuclei. MH27 outlines the fused hypodermal seam, which is seen as two parallel lines. (C) Hoechst staining of worm in B to show nuclei.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 10. RAB-6.2 and LIN-10 colocalize with RME-8 in neurons. (A-D) Fluorescence from neuron cell bodies (A and C) and ventral cord dendrites (B and D) is shown. (A-B'') Cerulean::RAB-6.2 (A and B) colocalizes with Venus::RME-8 (A' and B'; A'' and B'' show merged). (C-D'') LIN-10::CFP (C and D) colocalizes with Venus::RME-8 (C' and D'; C'' and D'' show merged). Arrowheads indicate colabeled puncta. Bars, 5 um.

Picture from Katsanos D et al. (2017) PLoS Biol "Stochastic loss and gain of symmetric divisions in the C. elegans epidermis ...."

(B) lin-22 smFISH in late L1 wild-type and lin-22(icb38)animals. In wild-type lin-22, spots were observed in anterior seam cells and not posterior(dashed line marks the seam cell boundary). (C) Quantification of lin-22 spots in the 4V1-V4 daughter cells early (n 11) and late (n 22) after the asymmetric division.

Picture from Reinhart BJ et al. (2001) Genetics "Isoform-specific mutations in the Caenorhabditis elegans heterochronic gene ...."

Figure 4. LIN-14 expression analysis. Animals were co-immunostained with antibodies to the common domain of the nuclear LIN-14 proteins (shown) as well as the monoclonal antibody MH27 (data not shown) to outline hypo- dermal cells for developmental stage determination. (A) LIN-14 expression is reduced in the LIN-14B null mutant lin-14(n360) by the mid-L1 stage, but the level of LIN-14 expression can be in- creased to almost wild-type levels in the double mutant lin-4(e912); lin-14(n360). (B) LIN-14A temporal misexpression is not maintained in lin-4(e912); lin-14(n360) animals. LIN-14 is barely detectable in a wild-type L2 stage animal (Ruvkun and Giusto 1989) although a lin-4(e912) L2 stage animal shows a high level of LIN-14 accumulation. lin-4(e912); lin-14(n360) animals accumulate slightly more LIN-14 than wild type but far less than lin-4(e912) alone.

Picture from Klassen MP et al. (2007) Cell "Wnt signaling positions neuromuscular connectivity by inhibiting synapse ...."

(A) Diagram representing the spatial relationship between lin-44 expressing cells (beige) and DA9 presynapses (red dots).(B) L2 lin-44(n1792) animal expressing a functional translational fusion of LIN-44::GFP (wyEx806). DA9 presynaptic terminals are labeled with mCherry::RAB-3 (wyEx677). Scale bar, 10 μm.

Picture from Katsanos D et al. (2017) PLoS Biol "Stochastic loss and gain of symmetric divisions in the C. elegans epidermis ...."

(A) Transgenic animals carrying transcriptionalreporters consisting of various fragments of upstream of lin-22 sequences fused to GFP. From top to bottom:full lin-22 endogenous promoter, distal lin-22 promoter region that is deleted in lin-22(icb38), proximal lin-22promoter present in lin-22(icb38), distal lin-22 promoter with deleted CR1, and CR1 only driving expression ofGFP. White arrows indicate expression in the seam cells; white arrowheads expression in the hypodermis andgreen arrowheads expression in intestinal cells. (B) Quantification of expression pattern for eachtranscriptional reporter (n=35 animals).