Picture from Liang J et al. (2003) Development "The Caenorhabditis elegans schnurri homolog sma-9 mediates stage- and cell ...."

sma-9 is widely expressed. sma-9 upstream sequences (K) were fused with a GFP reporter gene. (A-C) GFP expression in wild-type transgenic animals. The transgenic animals display a strong fluorescence in the VNC, pharynx and intestine, from stages L1 (not shown) to adult (A,B). L2 stage (C) animals show expression in the seam cells that disappears after L3 (Nomarski image in D). Arrows in C and D indicate seam cell nuclei. (E,I) Immunostaining by anti-SMA-9 antibodies against the unique 70 aa C terminus present in class II isoforms. (F,J) DAPI staining of the same animals. (E,F) N2; (I,J) enlarged view of the anterior region of a wild-type late L1 stage animal. In all animals, anterior is to the left, arrowheads show pharyngeal nuclei, black arrows show intestinal nuclei and white arrows show hypodermal nuclei. (K) Different sma-9 upstream regions used to construct GFP reporters.

Picture from Liang J et al. (2003) Development "The Caenorhabditis elegans schnurri homolog sma-9 mediates stage- and cell ...."

Figure 2. sma-9 is widely expressed. sma-9 upstream sequences (K) were fused with a GFP reporter gene. (A-C) GFP expression in wild-type transgenic animals. The transgenic animals display a strong fluorescence in the VNC, pharynx and intestine, from stages L1 (not shown) to adult (A,B). L2 stage (C) animals show expression in the seam cells that disappears after L3 (Nomarski image in D). Arrows in C and D indicate seam cell nuclei. (E,G,I) Immunostaining by anti-SMA-9 antibodies against the unique 70 aa C terminus present in class II isoforms. (F,H,J) DAPI staining of the same animals. (E,F) N2; (G,H) sma-9(wk55); (I,J) enlarged view of the anterior region of a wild-type late L1 stage animal. In all animals, anterior is to the left, arrowheads show pharyngeal nuclei, black arrows show intestinal nuclei and white arrows show hypodermal nuclei. (K) Different sma-9 upstream regions used to construct GFP reporters.

Picture from Inoue T et al. (2004) Proc Natl Acad Sci U S A "Type II platelet-activating factor-acetylhydrolase is essential for ...."

(I and J) paf-2::GFP expression in embryos (lateral view, dorsal is up). Nomarski micrographs (I) and corresponding paf-2::GFP expression (J). paf-2 reporter gene constructs are expressed in epidermal cells (J, arrowheads) and intestinal cells (J, arrow) at the beginning of elongation. Anterior is to the left. (Bar, 10 um.)

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.

Picture from Zhu Z et al. (2016) Dev Cell "Functional Coordination of WAVE and WASP in C.elegans Neuroblast ...."

(J-L) Fluorescence images (J) and quantification(K and L) of ARX-2 (GFP knockin). (J) Upper,merged image; middle, plasma membrane andhistone; lower, ARX-2. Arrows, leading edges;asterisks, ARX-2::GFP from an adjacent apoptoticcell. Box plots show the quantification of ARX2::GFP enrichment

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

I and J, nhx-3:: GFP expression in hypodermal cells (hyp).

Picture from Yin J et al. (2017) J Cell Biol "GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in ...."

(J-L") Confocal fluorescence images in wild type coexpressing GFP::GOP-1and markers of different organelles including Cherry::RAB-5 (J-J", early endosome), Cherry::RAB-7 (K-K", late endosome), or NUC-1::Cherry (L-L", lysosome). White arrowheads, vesicles with overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in closeproximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right (mean +- SD). Bars: (A-I') 10 um; (J-L") 5 um.

Picture from Munro E et al. (2004) Dev Cell "Cortical flows powered by asymmetrical contraction transport PAR proteins to ...."

(J-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-3.

Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

(J-K) GFP-tagged SER-1 is co-expressed with a cytosolic tdTomato in AVE in wild type (J), apb-3(ok429)(K) animals. Arrows point to the dendritic region of the AVE neuron. Scale bar is 10um.