FIGURE 5. Transcript levels of
unc-7 vary over the course of development. RNA was isolated from eggs (lane 1). the four larval stages LI (lane 2). L2 (lane 3).L3 (lane 4). and L4 (lane 5),and young adults(lane6). A northern blot containing total RNA, 30pg per lane, was hybridized to
unc-7 coding sequences (Ao) r an rRNA probe (B, showing 18s signal). Poly(A)-enriched RNA, 10 ug per lane (C), was hybridized to the same
unc-7 probe as in A.