Figure 3. Expression of
nsy-7::GFP and nuclear localization of NSY-7. (A) Expression of a 21-kb
nsy-7::GFP promoter fusion (green) and the AWC marker
odr-1::mCherry (red). The AWC cell body and AWC axons in the nerve ring are marked. (B)
nsy-7::GFP expression across development. (C) Nuclear localization of a NSY-7::GFP protein expressed in both AWCs under the
odr-3 promoter.
str-2::dsRed marks AWCON; note gain-of function 2-AWCON phenotype. (D) Cytoplasmic localization of NSY-7rGFP with the
ky630 mutation (
odr-3 promoter,
str-2::dsRed marker); note wild-type asymmetric expression of
str-2::dsRed. (E) Quantification of
str-2::dsRed expression in wild type, or wild type overexpressing
odr-3::
nsy-7::GFP with or without the
ky630 mutation. Numbers of animals scored are indicated.