Figure 3. Expression of the AAK-2::GFP fusion protein in C. elegans. The genomic DNA region including the 2.0 kb upstream of the start ATG and coding sequences of
aak-2 was fused to GFP, and a transgenic C. elegans line was generated. A, green fluorescence can be seen in the pharynx (Ph), excretory cell (Ex), vulva (Vul), and ventral cord (VC) of a whole adult worm. Green fluorescence was also present in body wall muscle (BWM) and in the cell bodies of the ventral cord (B), HSN neuron and vulva (V)(C), distal tip cell (DTC) and spermatheca (Sp)(D), and sheath cell (SC)(E). Scale bar, 50 μm. DIC, differential interference contrast; DAPI, 4',6-diamidino-2-phenylindole.