Picture from Labarre, Audrey et al. (2021) MicroPubl Biol

Figure 1. Expression of single copy transgenic human mutant FUS reproduces age-dependent ALS phenotypes in C. elegans: (A) Validation of human FUS transgene expression by qPCR (taqman). B) Adult worms were scored daily on solid media for paralysis phenotype. Transgenic mFUS worms show increased progressive paralysis over 12 days compared to transgenic wtFUS worms (p< 0.0001). (C) Expression of mFUS does not affect lifespan compared to wtFUS expression. (D) Synaptic cholinergic transmission has been evaluated by exposing day 1 adult worms to the cholinesterase inhibitor aldicarb. Worms were scored over 4 hours for aldicarb induced paralysis. Day 1 adult mFUS worms were hypersensitive to aldicarb treatment compared to either wtFUS or N2 (p< 0.0001). (E) Motility was further assessed in liquid M9 media by using the PhylumTech WMicrotrackerOne instrument. Adult day 1 mFUS worms have decreased motility capacity in liquid media over 10 hours compared to wtFUS and N2 (p< 0.0001). (F) Representative photo of an adult day 9 old unc-47p::mCherry;FUS worm with a degeneration event in the ventral neuronal processes of the GABAergic motor neurons (Highlighted in the magnified frame). (G) Quantification of neurodegeneration in transgenic worms at days 1, 5 and 9 of adulthood. At day 5 and 9 of adulthood mFUS worms show significantly higher neurodegeneration than wtFUS worms (p< 0.0001). (H) Representative photo of an adult day 9 old unc-47p::mCherry;mtFUS worm with a dorsal degeneration event in the axon of the GABAergic motor ­neurons (Highlighted in the magnified frame). (I) Quantification of dorsal axonal degeneration events in adult day 9 worms. Dorsal axonal degeneration can be observed more frequently in mFUS worms compared to wtFUS or unc-47p::mCherry control worms (p< 0.0001). (J) Representation of GABAergic neuronal health in the transgenic FUS populations. mFUS worms have a higher percentage of different degenerative events in the day 9 of adulthood (Ventral, dorsal or both ventro-dorsal degeneration).

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).

Picture from Liu H et al. (2006) Development "Direct regulation of egl-1 and of programmed cell death by the Hox protein MAB-5 ...."

CEH-20 is expressed in P11.aaap. DIC (A) and epifluorescence (B) images. (A,B) The posterior ventral nerve cord of a late L2 stage animal carrying a rescuing Pceh-20ceh-20:cfp fusion gene.